The Murine Lung Microbiome Changes During Lung Inflammation and Intranasal Vancomycin Treatment.
The Murine Lung Microbiome Changes During Lung Inflammation and Intranasal Vancomycin Treatment.
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DOI：
10.2174/1874285801509010167
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发表时间：
2015
期刊：
影响因子：
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通讯作者：
Krogfelt KA
中科院分区：
文献类型：
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作者：
Barfod KK;Vrankx K;Mirsepasi-Lauridsen HC;Hansen JS;Hougaard KS;Larsen ST;Ouwenhand AC;Krogfelt KA
大多数与气道疾病相关的微生物组研究都集中在肠道微生物组上。尽管培养独立微生物鉴定技术的进步表明，即使是健康的肺部也具有独特的动态微生物组。这种概念上的变化提出了一个问题；如果肺部疾病可能与局部肺部微生物群失调有因果关系。在这里，我们操纵小鼠的肺和肠道微生物群，以表明肺微生物群可以通过实验改变。我们使用了四种不同的方法：通过暴露于碳纳米管颗粒，口服益生菌和口服或鼻内暴露于抗生素万古霉素引起肺部炎症。从支气管肺泡和鼻灌洗液、盲肠样品中提取细菌DNA，并进行DGGE比较。我们的研究结果表明：肺部微生物群是性别依赖的，而不仅仅是肠道微生物群的反映，诱导炎症可以改变肺部微生物群。这种变化不会遗传给后代。成年小鼠口服益生菌不改变DGGE检测到的肺微生物组。鼻腔万古霉素可以优先改变肺部微生物群，而口服万古霉素则不会。这些观察结果应在未来研究肺部微生物群与肺部疾病之间的因果关系时予以考虑。
Most microbiome research related to airway diseases has focused on the gut microbiome. This is despite advances in culture independent microbial identification techniques revealing that even healthy lungs possess a unique dynamic microbiome. This conceptual change raises the question; if lung diseases could be causally linked to local dysbiosis of the local lung microbiota. Here, we manipulate the murine lung and gut microbiome, in order to show that the lung microbiota can be changed experimentally. We have used four different approaches: lung inflammation by exposure to carbon nano-tube particles, oral probiotics and oral or intranasal exposure to the antibiotic vancomycin. Bacterial DNA was extracted from broncho-alveolar and nasal lavage fluids, caecum samples and compared by DGGE. Our results show that: the lung microbiota is sex dependent and not just a reflection of the gut microbiota, and that induced inflammation can change lung microbiota. This change is not transferred to offspring. Oral probiotics in adult mice do not change lung microbiome detectible by DGGE. Nasal vancomycin can change the lung microbiome preferentially, while oral exposure does not. These observations should be considered in future studies of the causal relationship between lung microbiota and lung diseases.