Analysis of the cellular heterogeneity in the basal layer of mouse ear epidermis: an approach from partial decomposition in vitro and retroviral cell marking in vivo
Analysis of the cellular heterogeneity in the basal layer of mouse ear epidermis: an approach from partial decomposition in vitro and retroviral cell marking in vivo
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DOI：
10.1016/s0014-4827(02)00031-9
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发表时间：
2003-02-15
影响因子：
3.7
通讯作者：
Sugiyama, T
中科院分区：
文献类型：
--
作者：
Kameda, T;Nakata, A;Sugiyama, T
在薄表皮中，假设存在表皮增殖单位。每一个细胞单元都被分隔成每一个圆柱形的皮质板，估计在其基底层含有一个中央干细胞。我们试图验证这一假设在体外通过分析部分分解的小鼠耳表皮片段和体内使用逆转录病毒细胞标记。部分分解的小鼠耳表皮片段，主要由细胞角蛋白14表达的基底角质形成细胞组成，在体外形成多细胞集落。它们由形状不均匀的细胞组成，在形态上类似于每个单细胞衍生集落中的细胞，包括在体外具有巨大增殖潜能的潜在干细胞。碎片中干细胞候选者的估计频率远低于代表性假设的预测。逆转录病毒细胞标记与核定位LacZ蛋白在体内表明存在一个大的克隆细胞单位的表皮更新。从这些在体外和体内的观察，我们提出了一个新的模型的表皮增殖单位。(C)2003 Elsevier Science（美国）。All rights reserved.
In the thin epidermis, the existence of epidermal proliferation units was hypothesized. Each unit is supposed to be partitioned into each column of polygonal-shaped cornified plates, estimated to contain a central stem cell in its basal layer. We attempted to verify this hypothesis in vitro by analyzing the partially decomposed fragment of mouse ear epidermis and in vivo using retroviral cell marking. Partially decomposed fragments of the mouse ear epidermis, mostly composed of cytokeratin 14-expressing basal keratinocytes, formed multicellular colonies in vitro. They were composed of heterogeneously shaped cells, morphologically resembling the cells in each single cell-derived colony, including potential stem cells with great proliferative potency in vitro. The estimated frequency of the candidates of stem cells in the fragments was much lower than the prediction from the representative hypothesis. Retroviral cell marking with nuclear localizing LacZ protein in vivo suggested the existence of a large clonal cellular unit for epidermal renewal. From these in vitro and in vivo observations, we propose a new model for the epidermal proliferation unit. (C) 2003 Elsevier Science (USA). All rights reserved.