Candida Albicans Biofilm Formation on an Additive-Manufactured Titanium Alloy
Candida Albicans Biofilm Formation on an Additive-Manufactured Titanium Alloy
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增材制造钛合金上白色念珠菌生物膜的形成
DOI：
10.1007/978-3-031-22524-6_23
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发表时间：
2023
期刊：
影响因子：
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通讯作者：
Toru Okabe
中科院分区：
文献类型：
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作者：
Mari Koike;Tetsuro Horie;Susan K. Hummel;Richard J. Mitchell;Toru Okabe
[目的]比较不同制备方法对生物医用钛合金生物膜沉积的影响。方法采用3种制备方法：(1)采用电子束熔炼技术( )；(2)采用激光熔炼技术(EOSINT M270)；(3)利用离心铸造机(Ticast Super R)将熔蜡浇注到镁基包埋体中，制备直径为10 mm× 2 mm的Ti6Al-4V ELI合金(23级)。以Teflon®为对照。灭菌后，将每个标本(n = 3-6个/材料)放入井中并调理。白色念珠菌(CA)生物膜的形成采用Krom(2009年)描述的程序。将一毫升调整为光密度1的CAS悬浮液放置在每个样品上。在生物膜生长48h后，用生物发光法测定每个标本上的CA黏附活性。生物膜积累量以归一化到特氟龙®对照的数值表示。对每个试件的表面粗糙度进行了表征。实验进行了两次。结果虽然AM制备的钛合金表面粗糙度比铸态合金和α = ®对照组的表面粗糙度高，但AM合金表面的CAB膜聚集率与Teflon®无明显差异。然而，铸态钛合金表面的CAbiofm聚集量明显高于Teflon®对照组。结论在相对短暂的暴露后，钛合金表面的粗糙度似乎不影响CAbiofm的聚集量。
ObjectivesTo compare the effects of the fabrication method [additive-manufactured (AM) versus casting] on the biofilm accumulation on a biomedical titanium alloy.MethodsDisks (10 mm dia. × 2 mm thick) of Ti-6Al-4V ELI alloy (grade 23) were fabricated by three techniques: (1) electron beam melting (EBM) by AM (ArcamA2), (2) laser beam melting (LBM) by AM (EOSINT M270), and (3) lost-wax casting into a MgO-based investment using a centrifugal casting machine (Ticast Super R). Teflon®was used as a control. After sterilizing, each specimen (n = 3–6/material) was placed in a well and conditioned.Candida albicans (CA)biofilm was formed using procedures described by Krom (2009). One mL of theCAsuspension adjusted to optical density 1 was placed on each specimen. After 48 h of biofilm growth, the viability ofCAadhering to each specimen was determined using a bioluminescence assay. The biofilm accumulation is expressed as values normalized to those of the Teflon®controls. The surface roughness of each specimen was characterized. The experiment was performed twice. The data were analyzed using one-way ANOVA followed by Tukey’s test (α = 0.05).ResultsAlthough the surface roughnesses of Ti alloys fabricated by AM were rougher than the as-cast alloys and Teflon®controls, there was no difference between theCAbiofilm accumulation on the AM-fabricated alloys and the Teflon®. However, the as-cast Ti alloys accumulated significantly moreCAbiofilm than the Teflon®controls.ConclusionsAfter relatively brief exposure, surface roughness on Ti alloys appears to not affectCAbiofilm accumulation.