Contribution of PKC to beta-hexosaminidase-induced airway smooth muscle proliferation.
Contribution of PKC to beta-hexosaminidase-induced airway smooth muscle proliferation.
复制标题
PKC 对 β-己糖胺酶诱导的气道平滑肌增殖的贡献。
DOI：
10.1152/ajplung.1997.272.4.l639
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
Malik,KU
中科院分区：
文献类型：
--
作者：
Lew,DB;Brown,ER;Dempsey,BK;Wright,HM;Malik,KU
beta-Hexosaminidases (Hex) A and B promote mitogenesis via airway smooth muscle (ASM) mannose receptor. The objective of this study was to elucidate the contribution of protein kinase C (PKC) in Hex-induced mitogenesis in ASM cells (ASMC). Exposure of ASMC to Hex caused increases in both the calcium-dependent and the calcium-independent PKC activities. Both downregulation of PKC and PKC inhibitors staurosporine and calphostin C diminished Hex-induced DNA synthesis and cell number. Hex-induced DNA synthesis was enhanced by a diacylglycerol kinase inhibitor, R-59022, which was blocked by calphostin C. These data suggest that activation of PKC in part mediates Hex-induced mitogenesis in ASMC.
