Cloning and characterization of the Flavobacterium johnsoniae gliding motility genes gldD and gldE
Cloning and characterization of the Flavobacterium johnsoniae gliding motility genes gldD and gldE
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DOI：
10.1128/jb.183.14.4167-4175.2001
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发表时间：
2001-07-01
影响因子：
3.2
通讯作者：
McBride, MJ
中科院分区：
文献类型：
--
作者：
Hunnicutt, DW;McBride, MJ
约氏黄杆菌的细胞通过一种称为滑动运动的过程在表面上移动，这种运动形式的机制尚不清楚。 F.johnsoniae 细胞沿着其表面推动乳胶球，这被认为是运动机制的表现。最近描述了约氏杆菌滑翔运动所需的三个基因：gldA、gldB 和 ftsX。 Tn4351 诱变用于鉴定滑行所需的另一个基因 gldD。 Tn4351 诱导的 gldD 突变体形成非扩散集落，并且细胞无法滑动。它们还缺乏推动乳胶球的能力，并且对感染野生型细胞的噬菌体具有抵抗力。将野生型gldD引入突变体中可以恢复运动性、推动乳胶球的能力以及对噬菌体感染的敏感性。 gldD 编码的细胞质膜蛋白与已知功能的蛋白没有很强的序列相似性。 gldE 位于 gldD 的上游，编码另一种可能参与滑行运动的细胞质膜蛋白。gldE 的过表达部分抑制了 gldB 点突变体的运动缺陷，表明 GldB 和 GldE 可能相互作用。 GldE 与伯氏疏螺旋体 TlyC 和肠沙门氏菌血清型鼠伤寒 CorC 具有序列相似性。
Cells of Flavobacterium johnsoniae move over surfaces by a process known as gliding motility, The mechanism of this form of motility is not known. Cells of F.johnsoniae propel latex spheres along their surfaces, which is thought to be a manifestation of the motility machinery. Three of the genes that are required for F.johnsoniae gliding motility, gldA, gldB, and ftsX, have recently been described. Tn4351 mutagenesis was used to identify another gene, gldD, that is needed for gliding. Tn4351-induced gldD mutants formed nonspreading colonies, and cells failed to glide. They also lacked the ability to propel latex spheres and were resistant to bacteriophages that infect wild-type cells. Introduction of wild-type gldD into the mutants restored motility, ability to propel latex spheres, and sensitivity to bacteriophage infection. gldD codes for a cytoplasmic membrane protein that does not exhibit strong sequence similarity to proteins of known function. gldE, which lies immediately upstream of gldD, encodes another cytoplasmic membrane protein that may be involved in gliding motility, Overexpression of gldE partially suppressed the motility defects of a gldB point mutant, suggesting that GldB and GldE may interact. GldE exhibits sequence similarity to Borrelia burgdorferi TlyC and Salmonella enterica serovar Typhimurium CorC.