Expanded human cord blood-derived endothelial progenitor cells salvage infarcted myocardium in rats with acute myocardial infarction
Expanded human cord blood-derived endothelial progenitor cells salvage infarcted myocardium in rats with acute myocardial infarction
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DOI：
10.1111/j.1440-1681.2010.05347.x
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发表时间：
2010-05-01
影响因子：
2.9
通讯作者：
Wu, Gui-Fu
中科院分区：
文献类型：
--
作者：
Hu, Cheng-Heng;Li, Zhi-Ming;Wu, Gui-Fu
P>1。细胞移植有望成为急性心肌梗死（AMI）后恢复受损心脏功能的治疗选择。然而，使用的最佳细胞类型仍然存在争议。目的：1.探讨人脐血内皮祖细胞（hUCB-EPC）心肌内移植治疗大鼠AMI的疗效及可行性.结扎Wistar大鼠左前降支建立心肌梗死模型。标记的hUCB-EPC通过心肌内注射进行移植。使用压力-容积导管和超声心动图评估左心室功能。抗-VIII免疫组化染色用于通过计算平均毛细血管密度来反映梗死周围区域中的血管生成程度。Masson染色分析梗死心肌纤维化程度，计算胶原体积分数.用CM-Dil和FITC-UEA-1双阳性染色法在宿主心肌新生微血管中检测到标记的供体内皮祖细胞。左心室射血分数、左心室缩短分数、左心室收缩末期压力、左心室压力的一阶导数（+dP/dtmax和-dP/dtmax）的增加以及用细胞疗法的大鼠中左心室舒张末期压力的降低指示整体心脏功能的显著改善。与对照组相比，细胞治疗组的微血管形成增加，心肌纤维化程度降低。心肌纤维化程度较对照组轻.心肌梗死大鼠整体心功能的改善和心肌纤维化程度的降低提示hUCB-EPC移植的潜在益处。
P>1. Cell transplantation has promise as a therapeutic option for restoring impaired heart function after acute myocardial infarction (AMI). However, the optimal cell type to use remains controversial. We investigated the therapeutic efficacy and feasibility of intramyocardial transplantation of human umbilical cord blood-derived endothelial progenitor cells (hUCB-EPC) in rats with AMI.2. The Wistar rats myocardial infarction model was established by ligating the left anterior descending artery. The labelled hUCB-EPC were transplanted through intramyocardial injection. Left ventricular function was assessed using a pressure-volume catheter and echocardiogram. Anti-VIII immunohistochemistry staining was used to reflect the degree of angiogenesis in peri-infarcted areas by calculating the average capillary density. The fibrosis degree of infarcted myocardium was analysed by Masson staining and the collagen volume fraction was calculated.3. The labelled donor endothelial progenitor cells were detected in the new microvessels in host myocardium by double-positive staining with CM-Dil and FITC-UEA-l. An increase in left ventricular ejection fraction, left ventricular fractional shortening, left ventricular end-systolic pressure, first derivative of left ventricular pressure (+dP/dtmax and -dP/dtmax), as well as a decrease in the left ventricular end-diastolic pressure in rats with cell therapy indicated a significant improvement in global heart function. The cell therapy group had increased microvessel formation and a decreased degree of myocardial fibrosis compared to the control group. Moreover, the degree of myocardial fibrosis was less than that of the control group.4. The improved global heart function and decreased cardiac fibrosis in rats with AMI implies the potential benefit of hUCB-EPC transplantation.