Expression of the voltage-sensing phosphatase gene in the chick embryonic tissues and in the adult cerebellum.
Expression of the voltage-sensing phosphatase gene in the chick embryonic tissues and in the adult cerebellum.
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DOI：
10.4161/19420889.2014.970502
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发表时间：
2014-10
影响因子：
--
通讯作者：
Homma KJ
中科院分区：
文献类型：
--
作者：
Yamaguchi S;Aoki N;Kitajima T;Okamura Y;Homma KJ
电压敏感磷酸酶（VSP）由跨膜电压传感器结构域（VSD）和具有磷酸肌醇-磷酸酶活性的胞质结构域组成。它作为电压传感器，通过将VSD偶联到胞质结构域，直接将膜电位转化为磷酸肌醇周转。从海洋无脊椎动物到人类，VSP在进化上是保守的。最近，我们证明，异位表达的鸡直系同源的VSP，Gg-VSP，在成纤维细胞系引起的特征性细胞过程的产物。鸡PTEN的共表达抑制了这种形态学变化，表明VSP通过增加PI（3，4）P2来调节细胞形状。然而，Gg-VSP的体内功能仍不清楚。在这里，我们发现，在鸡胚Gg-VSP表达在胃，中肾，咽弓，肢芽，体节，神经管底板，脊索。此外，在小脑浦肯野神经元中发现了Gg-VSP转录本和蛋白。这些发现提供了一个深入了解VSP的生理功能。
Voltage-sensing phosphatase (VSP) consists of a transmembrane voltage sensor domain (VSD) and the cytoplasmic domain with phosphoinositide-phosphatase activities. It operates as the voltage sensor and directly translates membrane potential into phosphoinositide turnover by coupling VSD to the cytoplasmic domain. VSPs are evolutionarily conserved from marine invertebrate up to humans. Recently, we demonstrated that ectopic expression of the chick ortholog of VSP, Gg-VSP, in a fibroblast cell line caused characteristic cell process outgrowths. Co-expression of chick PTEN suppressed such morphological change, suggesting that VSP regulates cell shape by increasing PI(3,4)P2. However, the in vivo function of Gg-VSP remains unclear. Here, we showed that in chick embryos Gg-VSP is expressed in the stomach, mesonephros, pharyngeal arch, limb bud, somites, floor plate of neural tube, and notochord. In addition, both Gg-VSP transcripts and the protein were found in the cerebellar Purkinje neurons. These findings provide an insight into the physiological functions of VSP.