The Nicotiana chloroplast genome. IX. Identification of regions active as prokaryotic promoters in Escherichia coli.
The Nicotiana chloroplast genome. IX. Identification of regions active as prokaryotic promoters in Escherichia coli.
复制标题
烟草叶绿体基因组。
DOI：
10.1016/0378-1119(84)90191-4
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发表时间：
1984
期刊：
影响因子：
3.5
通讯作者：
S. Kung
中科院分区：
文献类型：
--
作者：
X. Kong;P. S. Lovett;S. Kung
galK 表达质粒载体系统 pKO1 已用于克隆在大肠杆菌中发挥作用的烟草叶绿体 (ct) 启动子。随机克隆的含有启动子的限制性片段位于 ct 基因组上，并且源自编码核糖体和转移 RNA 的区域以及 ct 基因组上其他位置。该结果首次证明了ct基因组中存在具有原核启动子功能的序列。
ThegalK-expression plasmid vector system pKO1 has been used to cloneNicotianachloroplast (ct) promoters that function inEscherichia coli. The randomly cloned promoter-containing restriction fragments have been located on the ct genome and originate both from those regions encoding ribosomal and transfer RNAs and from locations elsewhere on the ct genome. The results provide the first demonstration that sequences which function as prokaryotic promoters exist in the ct genome.