DECLINE OF ACTIVITY AND QUANTITY OF RIBULOSE BISPHOSPHATE CARBOXYLASE OXYGENASE AND NET PHOTOSYNTHESIS IN OZONE-TREATED POTATO FOLIAGE
DECLINE OF ACTIVITY AND QUANTITY OF RIBULOSE BISPHOSPHATE CARBOXYLASE OXYGENASE AND NET PHOTOSYNTHESIS IN OZONE-TREATED POTATO FOLIAGE
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DOI：
10.1104/pp.91.1.427
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发表时间：
1989-09-01
期刊：
影响因子：
7.4
通讯作者：
PELL, EJ
中科院分区：
文献类型：
--
作者：
DANN, MS;PELL, EJ
研究了臭氧（O3）对温室栽培龙葵（Solanum tuberosum L. cv " Norland "）叶片二磷酸核酮糖羧化酶/加氧酶（Rubisco）活性、数量和净光合作用的影响与氧化性早衰的关系。在受控环境室中，从1000小时到1600小时，将26天的植物暴露于0.06至0.08微升/升的O3中，持续4天。在第5天，植物暴露于6小时的模拟倒温中，其中O3最高为0.12微升/升。净光合作用对O3的响应下降，但在暴露结束3天后恢复到接近对照的水平。对照马铃薯叶片Rubisco活性和数量在12 d内先上升后下降。在一些实验中，所研究的叶片在生理上成熟，Rubisco活性在O3暴露开始时达到峰值。在这些情况下，O3加速了Rubisco活性的下降。当不成熟的叶片被O3处理时，叶片的Rubisco活性从未达到对照叶片的最高水平，并且表现出更快的初始和总活性下降。Rubisco的激活百分比（初始/总活性）不受治疗的显著影响。Rubisco的数量随活性的降低而降低。活性的降低很可能是由于可用蛋白质的减少，而不是体内活化Rubisco百分比的减少。Rubisco是一种重要的叶面贮藏蛋白，其数量的减少可能与这种空气污染物的毒性有关。
The effect of ozone (O3) on ribulose bisphosphate carboxylase/oxygenase (Rubisco) activity and quantity and net photosynthesis in greenhouse-grown Solanum tuberosum L. cv ''Norland'' foliage was studied in relation to oxidant-induced premature senescence. Plants, 26 days old, were exposed to 0.06 to 0.08 microliters per liter O3 from 1000 to 1600 hours for 4 days in a controlled environment chamber. On day 5, plants were exposed to a 6-hour simulated inversion in which O3 peaked at 0.12 microliters per liter. Net photosynthesis declined in response to O3 but recovered to near control levels 3 days after the exposure ended. Rubisco activity and quantity in control potato foliage increased and then decreased during the 12-day interval of the study. In some experiments foliage studied was physiologically mature and Rubisco activity had peaked when O3 exposure commenced. In those cases, O3 accelerated the decline in Rubisco activity. When less mature foliage was treated with O3, the leaves never achieved the maximal level of Rubisco activity observed in control foliage and also exhibited more rapid decline in initial and total activity. Percent activation of Rubisco (initial/total activity) was not affected significantly by treatment. Quantity of Rubisco decreased in concert with activity. The decrease in activities is most likely due to a decrease in available protein rather than a decrease in the percentage of Rubisco activated in vivo. The reduction in the quantity of Rubisco, an important foliar storage protein, could contribute to premature senescence associated with toxicity of this air pollutant.