The response of the fibrinolytic system to mycobacteria infection
The response of the fibrinolytic system to mycobacteria infection
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DOI：
10.1016/j.tube.2012.07.002
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发表时间：
2012-11-01
期刊：
影响因子：
3.2
通讯作者：
Espitia, Clara
中科院分区：
文献类型：
--
作者：
Rodriguez-Flores, Eden;Campuzano, Jaime;Espitia, Clara
人类病原体结核分枝杆菌与多种宿主细胞蛋白结合，包括纤维蛋白溶解系统的蛋白。这些观察结果促使我们在进行性肺结核的动物模型中研究该系统的组分的表达。BALB/c小鼠感染M.分析结核病H37 Rv的纤溶酶/纤溶酶原和组织纤溶酶原激活物的表达和酶活性，以及纤溶酶原、组织和尿激酶纤溶酶原激活物的mRNA水平。免疫组化法检测到纤溶酶原。结果表明，纤维蛋白溶解系统的分子的表达在感染过程中逐渐增加，在疾病的慢性期达到峰值。体外实验表明，卡介苗免疫小鼠脾细胞经M.结核蛋白总之，这些结果表明，纤维蛋白溶解系统的分子在实验性结核病的慢性期上调，并表明分枝杆菌本身可能在纤维蛋白溶解系统的分子的过度表达中发挥重要作用，从而导致结核病的慢性炎症。(C)2012爱思唯尔有限公司保留所有权利。
The human pathogen Mycobacterium tuberculosis binds to a variety of host cell proteins, including those of the fibrinolytic system. These observations prompted us to study the expression of components of this system in an animal model of progressive pulmonary tuberculosis. Lung homogenates from BALB/c mice infected with M. tuberculosis H37Rv were analyzed to determine the expression and enzymatic activity of plasmin/plasminogen and tissue plasminogen activator, as well as the mRNA levels for plasminogen, tissue and urokinase plasminogen activators. Plasminogen was also detected in infected lungs with immunohistochemistry. The results show that the expression of molecules of the fibrinolytic system increased gradually over the course of the infection, peaking during the chronic phase of the disease. Furthermore, in vitro experiments showed that both plasminogen activators were specifically induced after the stimulation of spleen cells from BCG-immunized mice with M. tuberculosis proteins. Together, these results show that molecules of the fibrinolytic system are up-regulated in the chronic phase of experimental tuberculosis and suggest that the mycobacterium itself could play an important role in the overexpression of molecules of the fibrinolytic system, contributing to chronic inflammation in tuberculosis. (C) 2012 Elsevier Ltd. All rights reserved.