Blood glucose-lowering effect of water and ethanolic extracts of Gynura procumbens (Lour.) Merr
Blood glucose-lowering effect of water and ethanolic extracts of Gynura procumbens (Lour.) Merr
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Gynura procumbens (Lour.) Merr 的水和乙醇提取物的降血糖作用
DOI：
10.1002/tkm2.1277
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Maruyama Hiroko
中科院分区：
文献类型：
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作者：
Aung Cho L.;Kawakami Fumitaka;Imai Motoki;Lwin Thet‐Thet;Kanzaki Makoto;Mar Ohn;Phyu Khin P.;Thwin Mya M.;Maruyama Hiroko
AimGynura proumbens(Lour.)默尔。(GP)作为治疗糖尿病的替代药物已经引起了人们的注意。然而，以往的研究大多是在糖尿病小鼠身上进行的，本研究对GP对饮食诱导的肥胖小鼠的降血糖作用进行了研究，并阐明了GP对肌肉和脂肪细胞的降血糖作用的可能机制。方法将小鼠分为4组(每组6只)，分别给予正常饮食(ND)、正常饮食 + 1%GP粉(ND + GP)、高脂饮食(高脂饮食)、高脂饲料高脂饲料1%糖蛋白粉组( + + GP组)，分别于1、3、5个月后进行口服糖耐量试验。免疫细胞化学显示GLUT4易位于稳定表达myc-GLUT4-ECFP的细胞。用免疫印迹法检测GP提取物对C2C12(肌肉细胞)和3T3-L1(脂肪细胞)细胞GLUT4表达及5‘-腺苷一磷酸活化蛋白激酶(AMPK)和Akt/蛋白激酶B(PKB)磷酸化的影响。此外，与3T3-L1细胞相比，GP提取物能更显著地促进C2C12细胞的GLUT4膜转位，而GLUT4的表达仅在3T3-L1细胞中显著增加。两种提取物均能显著提高C2C12细胞和3T3-L1细胞的AMPK磷酸化水平。结论GP具有纠正糖耐量异常的作用，GP醇提物和水提物均有降血糖作用，其作用机制可能与肌肉和脂肪组织中AMPK信号转导途径有关。
AimGynura procumbens(Lour.) Merr. (GP) has drawn attention as an alternative drug for the treatment of diabetes mellitus. However, while most previous studies were done in diabetic mice to investigate the antidiabetic effect, this study investigated the blood glucose‐lowering action of GP in diet‐induced obese mice and clarified the possible mechanism of the glucose‐lowering action of GP in muscle and adipose cells.MethodsThe mice were divided into four groups (n= 6 in each group) depending on the diet that they received (i.e., the normal diet (ND), normal diet + 1% GP powder (ND + GP), high‐fat diet (HFD), high‐fat diet + 1% GP powder (HFD + GP) groups), and the oral glucose tolerance test (OGTT) was performed after one, three and five months. Immunocytochemistry revealed GLUT4 translocation in cells stably expressing myc‐GLUT4‐ECFP. The effect of GP extracts on the expression of GLUT4 and phosphorylation of 5'‐adenosine monophosphate‐activated protein kinase (AMPK) and Akt/protein kinase B (PKB) in C2C12 (muscle cell) and 3T3‐L1 (adipocyte) cells, was determined by western blot analysis.ResultsThe results showed that the significantly raised serum glucose levels in the HFD group were reduced following treatment with GP powder. Moreover, treatment with the GP extracts promoted GLUT4 membrane translocation more prominently in the C2C12 cells as compared to the 3T3‐L1 cells, whereas GLUT4 expression was significantly increased only in the 3T3‐L1 cells. AMPK phosphorylation was significantly increased by both extracts in both C2C12 cells and 3T3‐L1 cells.ConclusionThe findings of the present study reveal that GP may correct glucose intolerance and both the ethanolic and water extracts of GP have a blood glucose‐lowering effect, possibly via AMPK‐mediated signaling pathways in the muscle and adipose tissues.