Transcription profiling of fascistic acid related pathways in SNAC4/9 silenced tomato fruits
Transcription profiling of fascistic acid related pathways in SNAC4/9 silenced tomato fruits
复制标题
SNAC4/9 沉默番茄果实中法西斯酸相关途径的转录谱
DOI：
10.1007/s12209-020-00262-8
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发表时间：
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影响因子：
7.1
通讯作者：
Kou xiaohong
中科院分区：
文献类型：
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作者：
liu yefang;Kou xiaohong
NAC（NAM、ATAF和CUC）家族被认为是植物转录因子中最大的家族之一，其成员参与果实成熟。脱落酸（阿坝）已被证明可以调节果实成熟过程。利用病毒诱导的基因沉默方法和新一代测序技术，比较分析了SNAC 4（SlNAC 48，登录号：NM 001279348.2）和SNAC 9（SlNAC 19，登录号：XM 004236996.2）对番茄果实成熟的影响。高通量测序结果表明，SNAC 4沉默的果实与对照果实相比，鉴定出1262个显著差异表达基因（DEG），而SNAC 9沉默的果实鉴定出655个DEG。此外，我们还分别在< 0.05 and log2-fold change >SNAC 4沉默和SNAC 9沉默的番茄中选择了26个和30个与阿坝相关的显著DEG（p1.0）进行进一步分析。在SNAC 4沉默的番茄中，XET基因和另外两个基因（E8和EXP 1）分别显著下调和上调。然而，PYL 9基因和其他四个基因（PP 2C，CYP 707 A2，EXPA 6和ACS 6）在SNAC 9沉默的番茄中分别显著下调和上调。此外，选择了10个DEG用于试验，以通过定量实时聚合酶链反应（qRT-PCR）确认转录组学结果的准确性。研究结果揭示了SNAC 4/9和阿坝在番茄成熟调控中的相互关系，为进一步研究番茄果实成熟衰老机制提供了理论依据。
The NAC (NAM, ATAF, and CUC) family is considered one of the largest families of plant transcription factor, and its members are involved in fruit ripening. Abscisic acid (ABA) has been demonstrated to modulate the fruit ripening process. By applying the virus-induced gene silencing method and next-generation sequencing technology, we conducted a comparative analysis of the effects ofSNAC4(SlNAC48, accession number: NM 001279348.2) andSNAC9(SlNAC19, accession number: XM 004236996.2) on tomato fruit ripening. The results of high-throughput sequencing identified 1262 significant (p< 0.05) differentially expressed genes (DEGs) inSNAC4-silenced fruit compared to control fruit, while 655 DEGs were identified inSNAC9-silenced fruit. In addition, we selected 26 and 30 significant DEGs (p< 0.05 and log2-fold change > 1.0) related to ABA inSNAC4-silenced andSNAC9-silenced tomatoes, respectively, for further analysis. TheXETgene and two other genes (E8andEXP1) were significantly down and upregulated inSNAC4-silenced tomatoes, respectively. However, thePYL9gene and four other genes (PP2C,CYP707A2,EXPA6, andACS6) were significantly down and upregulated inSNAC9-silenced tomatoes, respectively. In addition, ten DEGs were selected for use in tests to confirm the accuracy of the transcriptomic results by quantitative real-time polymerase chain reaction (qRT-PCR). Our results highlight the relationship between SNAC4/9 and ABA in the regulation of tomato ripening, which may help provide a theoretical basis for further research on the mechanisms of tomato fruit ripening and senescence.