In vivo mesenchymal cell recruitment by a scaffold loaded with transforming growth factor β1 and the potential for in situ chondrogenesis
In vivo mesenchymal cell recruitment by a scaffold loaded with transforming growth factor β1 and the potential for in situ chondrogenesis
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DOI：
10.1089/107632702760184727
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发表时间：
2002-06-01
影响因子：
--
通讯作者：
Lee, EH
中科院分区：
文献类型：
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作者：
Huang, Q;Hutmacher, DW;Lee, EH
本研究的目的是（1）开发一种由生物可吸收聚合物支架与载TGF-β 1的纤维蛋白胶组合制成的双相植入物，用于组织工程应用，以及（2）确定由聚己内酯（PCL）支架和载TGF-β 1的纤维蛋白胶制成的植入物是否可以招募间充质细胞，并在植入异位部位时诱导软骨形成过程。使用24只6月龄新西兰白色家兔。将在纤维蛋白胶中负载不同剂量TGF-β 1的支架植入皮下、肌肉内和骨膜下。术后2、4、6周处死兔，取出种植体。对标本进行各种染色技术进行组织学分析。所有样品的光学显微镜检查显示，在每组中，支架的整个孔隙空间被各种组织填充。在负载有TGF-β 1并肌内和皮下植入的组中，支架的整个体积充满了间充质细胞，周围有丰富的细胞外基质和血管。发现加载有TGF-β 1并骨膜下植入的支架在2周和4周时富含软骨细胞，并且在6周时鉴定出未成熟骨形成。我们的结论是，载TGF-β 1的支架可以成功地招募间充质细胞和软骨发生时，这种结构植入骨膜下。
The objectives of this study were (1) to develop a biphasic implant made of a bioresorbable polymeric scaffold in combination with TGF-beta1-loaded fibrin glue for tissue-engineering applications, and (2) to determine whether the implant made of a polycaprolactone (PCL) scaffold and TGF-beta1-loaded fibrin glue could recruit mesenchymal cells and induce the process of cartilage formation when implanted in ectopic sites. Twenty-four 6-month-old New Zealand White rabbits were used. Scaffolds loaded with various doses of TGF-beta1 in fibrin glue were implanted subcutaneously, intramuscularly, and subperiosteally. The rabbits were killed and implants were removed at 2, 4, and 6 weeks postoperatively. The specimens were subjected to various staining techniques for histological analysis. Light microscopic examination of all specimens revealed that the entire pore space of the scaffolds was filled with various tissues in each group. The entire volume of the scaffolds in the groups loaded with TGF-beta1 and implanted intramuscularly and subcutaneously was populated with mesenchymal cells surrounded with an abundant extracellular matrix and blood vessels. The scaffold loaded with TGF-beta1 and implanted subperiosteally was found to be richly populated with chondrocytes at 2 and 4 weeks and immature bone formation was identified at 6 weeks. We conclude that scaffolds loaded with TGF-beta1 can successfully recruit mesenchymal cells and that chondrogenesis occurred when this construct was implanted subperiosteally.