Improved reproducibility and quantitative analysis of phospholipids by flame ionization detection.
Improved reproducibility and quantitative analysis of phospholipids by flame ionization detection.
复制标题
通过氢火焰离子化检测提高了磷脂的重现性和定量分析。
DOI：
10.1016/0021-9673(85)80036-4
复制
发表时间：
1985
期刊：
影响因子：
--
通讯作者：
Murray,DK
中科院分区：
文献类型：
--
作者：
Murray,DK
通过用汉密尔顿注射器小心地应用样品和使用密封的双罐系统，改进了通过火焰离子化的磷脂定量分析。如果在密封的双槽系统中进行显影，则色棒的显影与可再现的扫描时间或RF值（变异系数为1%）更一致，并且峰更尖锐。将Chromarod置于内槽中，该内槽是配备有Iatroscan TH-10系统的标准毛玻璃顶槽。将该内罐置于较大的薄层色谱罐内，该薄层色谱罐用硅脂密封，并用铅砖将盖固定到位。两个罐都衬有吸水纸，并含有相同的溶剂系统。用这些程序定量并以1至30 μg的量测量的生物样品的变异系数在0.2%至6%之间。本文介绍了一种能将中性脂质与磷脂完全分离并能定量测定胆固醇的有效方法。比较各种磷脂的扫描时间和RF值，以确定在3 T3-L1和白细胞膜中发现的主要磷脂的最佳分离。
Quantitative analysis of phospholipids by flame ionization was improved by careful application of samples with a Hamilton syringe and use of a sealed dual tank system. Chromarods developed more consistently with reproducible scanning times orRFvalues (coefficient of variation of 1%) and with sharper peaks if development was carried out in a sealed dual tank system. The Chromarods were placed in the inner tank, which was the standard ground glass topped tank furnished with the Iatroscan TH-10 system. This inner tank was placed inside a larger thin-layer chromatography tank which was sealed with silicone grease and the lid held in place with a lead brick. Both tanks were lined with absorbent paper and contained the same solvent system. Biological samples quantified with these procedures and measured in amounts between 1 and 30 μg had coefficients of variation between 0.2 and 6%. An efficient method of completely separating neutral lipids from phospholipids and allowing quantitative determination of cholesterol is desribed. Scanning times andRFvalues of various phospholipids are compared to determine the best separation of the major phospholipids found in 3T3-L1 and leukocyte membranes.