Prion protein library of recombinant constructs for structural biology
Prion protein library of recombinant constructs for structural biology
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DOI：
10.1111/j.1742-4658.2009.06968.x
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发表时间：
2009-04-01
期刊：
影响因子：
5.4
通讯作者：
Wuethrich, Kurt
中科院分区：
文献类型：
--
作者：
Hornemann, Simone;Christen, Barbara;Wuethrich, Kurt
调查的质粒为51朊病毒蛋白的构建体从银行田鼠，猫，牛，鸡，狗，麋鹿，雪貂，青蛙，河豚，马，人，猪，羊，龟，和小袋鼠，和为113小鼠朊病毒蛋白的构建体及其变体。这包括重组蛋白的生物化学信息，特别是成功和不成功的表达尝试。质粒文库是在我们实验室的朊病毒蛋白的NMR结构测定和生物物理表征的背景下在过去12年中产生的。这些质粒现在可供普遍使用，并免费分发给非营利机构。
A survey of plasmids for 51 prion protein constructs from bank vole, cat, cattle, chicken, dog, elk, ferret, frog, fugu, horse, human, pig, sheep, turtle, and wallaby, and for 113 mouse prion protein constructs and variants thereof, is presented. This includes information on the biochemistry of the recombinant proteins, in particular on successful and unsuccessful expression attempts. The plasmid library was generated during the past 12 years in the context of NMR structure determination and biophysical characterization of prion proteins in our laboratory. The plasmids are now available for general use, and are distributed free of charge to not-for-profit institutions.