Hydrostatic pressure influences mRNA expression of transforming growth factor-beta 1 and heat shock protein 70 in chondrocyte-like cell line
Hydrostatic pressure influences mRNA expression of transforming growth factor-beta 1 and heat shock protein 70 in chondrocyte-like cell line
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DOI：
10.1002/jor.1100150122
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发表时间：
1997-01-01
影响因子：
2.8
通讯作者：
Hirasawa, Y
中科院分区：
文献类型：
--
作者：
Takahashi, K;Kubo, T;Hirasawa, Y
本研究探讨了静水压力对软骨细胞样细胞系中细胞因子和热休克蛋白70表达的影响。软骨细胞样细胞（HCS-2/8）暴露于静水压力通过一个特殊的压力装置。提取细胞因子（白细胞介素-1 β、碱性成纤维细胞生长因子、胰岛素样生长因子-I和转化生长因子-β 1）和热休克蛋白70的总RNA，并通过聚合酶链反应法和北方印迹法进行分析。进行了[S-35]硫酸盐掺入试验，以评估蛋白聚糖合成。转化生长因子-β 1 mRNA的表达增强后，暴露于5 MPa的静水压力和降低后，而热休克蛋白70的表达增强后，暴露于50 MPa的静水压力。[S-35]硫酸盐掺入培养的细胞增加暴露于1-5 MPa的静水压力和10-50 MPa的压力后下降。这些结果表明，在生理水平的静水压力增强转化生长因子-β 1 mRNA的表达，除了增加蛋白多糖在软骨细胞中的合成，过高的静水压力降低转化生长因子-β 1 mRNA的表达和增加热休克蛋白70 mRNA的表达，同时减少蛋白多糖的合成。
The present study investigated the influence of hydrostatic pressure on the expression of cytokines and heat shock protein 70 in a chondrocyte-like cell line. Chondrocyte-like cells (HCS-2/8) were exposed to hydrostatic pressure by a special pressure apparatus. Total RNA for cytokines (interleukin-1 beta, basic fibroblast growth factor, insulin-like growth factor-I, and transforming growth factor-beta 1) and for heat shock protein 70 was extracted and was analyzed by a polymerase chain reaction method and Northern blotting. An assay for incorporation of [S-35]sulfate was performed to assess proteoglycan synthesis. The expression of transforming growth factor-beta 1 mRNA was enhanced after exposure to 5 MPa of hydrostatic pressure and was reduced after 50 MPa, whereas the expression of heat shock protein 70 was enhanced following exposure to 50 MPa of hydrostatic pressure. The incorporation of [S-35]sulfate into the cultured cells increased following exposure to 1-5 MPa of hydrostatic pressure and decreased following 10-50 MPa of pressure. These results suggest that hydrostatic pressure at physiologic levels enhances the expression of transforming growth factor-beta 1 mRNA in addition to increasing proteoglycan synthesis in chondrocytes and that excessively high hydrostatic pressure reduces the expression of transforming growth factor-beta 1 mRNA and increases the expression of heat shock protein 70 mRNA while decreasing proteoglycan synthesis.