3-DIMENSIONAL SPECTRAL SPATIAL EPR IMAGING OF FREE-RADICALS IN THE HEART - A TECHNIQUE FOR IMAGING TISSUE METABOLISM AND OXYGENATION
3-DIMENSIONAL SPECTRAL SPATIAL EPR IMAGING OF FREE-RADICALS IN THE HEART - A TECHNIQUE FOR IMAGING TISSUE METABOLISM AND OXYGENATION
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DOI：
10.1073/pnas.91.8.3388
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发表时间：
1994-04-12
影响因子：
11.1
通讯作者：
ZWEIER, JL
中科院分区：
文献类型：
--
作者：
KUPPUSAMY, P;CHZHAN, M;ZWEIER, JL
据推测，活体器官和组织（如心脏）中的自由基代谢和氧合可能会随着空间定义的组织结构而变化。为了研究这些空间上的差异，我们开发了电子顺磁共振成像仪器，可以对注入心脏和大血管的自由基进行三维光谱空间成像。使用该仪器，可以获得离体灌注大鼠心脏和兔主动脉的高质量三维光谱空间图像。在离体主动脉中，可以获得活体血管组织中血管腔和血管壁的空间和光谱精确图像。在离体大鼠心脏中，通过影像学实验确定心肌缺血时心脏不同空间位置自由基清除的动力学。动力学数据显示心肌自由基清除存在区域性和跨壁性差异。进一步证明EPR成像可用于无创测量心脏空间局部氧浓度。因此，光谱空间EPR成像技术被证明是一种强有力的工具，可以提供有关活体生物器官和组织中自由基分布、代谢和组织氧合的空间信息。
It has been hypothesized that free radical metabolism and oxygenation in living organs and tissues such as the heart may vary over the spatially defined tissue structure. In an effort to study these spatially defined differences, we have developed electron paramagnetic resonance imaging instrumentation enabling the performance of three-dimensional spectral-spatial images of free radicals infused into the heart and large vessels. Using this instrumentation, high-quality three-dimensional spectral-spatial images of isolated perfused rat hearts and rabbit aortas are obtained. In the isolated aorta, it is shown that spatially and spectrally accurate images of the vessel lumen and wall could be obtained in this living vascular tissue. In the isolated rat heart, imaging experiments were performed to determine the kinetics of radical clearance at different spatial locations within the heart during myocardial ischemia. The kinetic data show the existence of regional and transmural differences in myocardial free radical clearance. It is further demonstrated that EPR imaging can be used to noninvasively measure spatially localized oxygen concentrations in the heart. Thus, the technique of spectral-spatial EPR imaging is shown to be a powerful tool in providing spatial information regarding the free radical distribution, metabolism, and tissue oxygenation in living biological organs and tissues.