Proliferation and Migration of Label-Retaining Cells of the Kidney Papilla
Proliferation and Migration of Label-Retaining Cells of the Kidney Papilla
复制标题
DOI：
10.1681/asn.2008111203
复制
发表时间：
2009-11-01
影响因子：
13.6
通讯作者：
Al-Awqati, Qais
中科院分区：
文献类型：
--
作者：
Oliver, Juan A.;Klinakis, Apostolos;Al-Awqati, Qais
肾乳头包含一群具有成体干细胞若干特征的细胞，包括在长时间追逐期间保留增殖标记物（即，它们是标记保留细胞[lrc]）。为了确定乳头状lrc是否在正常成人肾脏中产生新细胞，我们检查了整个肾脏的细胞增殖情况，发现上乳头是细胞循环增强的部位。使用有条件地表达与组蛋白213融合的绿色荧光蛋白的转基因小鼠，我们观察到乳头的LRCs仅在其上部增殖，在那里它们与循环细胞的“链”相关联。乳头状lrc的数量随着年龄的增长而减少，表明细胞在进入细胞周期之前迁移到上乳头。为了直接验证这一点，我们在活体中用重要的染料标记了乳头状细胞，发现肾乳头中的一些细胞，包括LRCs，向肾脏的其他部位迁移。急性肾损伤增强了细胞的迁移和增殖。这些结果表明，在正常的稳态状态下，肾乳头（或其直系后代）的lrc迁移到上乳头并形成快速增殖的细胞室，这可能在缺血性损伤后的修复中发挥作用。
The kidney papilla contains a population of cells with several characteristics of adult stem cells, including the retention of proliferation markers during long chase periods (i.e., they are label-retaining cells [LRCs]). To determine whether the papillary LRCs generate new cells in the normal adult kidney, we examined cell proliferation throughout the kidney and found that the upper papilla is a site of enhanced cell cycling. Using genetically modified mice that conditionally expressed green fluorescence protein fused to histone 213, we observed that the LRCs of the papilla proliferated only in its upper part, where they associate with "chains" of cycling cells. The papillary LRCs decreased in number with age, suggesting that the cells migrated to the upper papilla before entering the cell cycle. To test this directly, we marked papillary cells with vital dyes in vivo and found that some cells in the kidney papilla, including LRCs, migrated toward other parts of the kidney. Acute kidney injury enhanced both cell migration and proliferation. These results suggest that during normal homeostasis, LRCs of the kidney papilla (or their immediate progeny) migrate to the upper papilla and form a compartment of rapidly proliferating cells, which may play a role in repair after ischemic injury.