CX3CL1 expression induced by Candida albicans in oral fibroblasts
CX3CL1 expression induced by Candida albicans in oral fibroblasts
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DOI：
10.1111/j.1574-695x.2010.00734.x
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发表时间：
2010-11-01
影响因子：
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通讯作者：
Kamata, Nobuyuki
中科院分区：
文献类型：
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作者：
Ohta, Kouji;Nishi, Hiromi;Kamata, Nobuyuki
口腔成纤维细胞以及角化细胞被认为影响宿主对白色念珠菌的炎症反应。然而，对口腔成纤维细胞中抗白色念珠菌感染的趋化因子表达知之甚少。因此，我们研究了白色念珠菌是否在成纤维细胞和角质形成细胞中诱导了几种趋化因子，包括fractalkine/CX3CL1 (CX3CL1)，这是一种独特的趋化因子，具有趋化剂和粘附分子的特性。将白色念珠菌活细胞添加到人永生化口腔角质形成细胞（RT7）中，导致多种趋化因子mRNA水平升高，但CX3CL1没有升高。相比之下，活的和热灭的白色念珠菌引起人永生化口腔成纤维细胞（GT1）中CX3CL1 mRNA和蛋白表达的增加。CX3CL1 mRNA在GT1细胞中的表达也在非白色念珠菌的刺激下增强。此外，CX3CL1趋化因子结构域对白色念珠菌具有抗真菌活性。口腔成纤维细胞分泌的CX3CL1似乎在口腔对白色念珠菌感染的免疫应答中起重要作用。
Oral fibroblasts as well as keratinocytes are thought to influence host inflammatory responses against Candida albicans. However, little is known about chemokine expressions in oral fibroblasts against C. albicans infection. We therefore examined whether C. albicans induced several chemokines including fractalkine/CX3CL1 (CX3CL1), a unique chemokine that has properties of both chemoattractants and adhesion molecules, in fibroblasts and keratinocytes. The addition of C. albicans live cells to human immortalized oral keratinocytes (RT7) resulted in increases in the mRNA levels of multiple chemokines, but not of CX3CL1. In contrast, live and heat-killed C. albicans caused an increase in CX3CL1 mRNA and protein expression in human immortalized oral fibroblasts (GT1). CX3CL1 mRNA expression in GT1 cells was also enhanced by stimulation with a nonalbicans species of Candida. Further, the CX3CL1 chemokine domain showed antifungal activity against C. albicans. CX3CL1 secreted by oral fibroblasts appears to play an important role in the oral immune response to C. albicans infection.