Recording the ventilation activity of free-swimming zebrafish and its application to novel tank tests
Recording the ventilation activity of free-swimming zebrafish and its application to novel tank tests
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记录自由游动斑马鱼的通气活动及其在新型水箱测试中的应用
DOI：
10.1016/j.physbeh.2021.113665
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发表时间：
2022
影响因子：
2.9
通讯作者：
Yoshida Masayuki
中科院分区：
文献类型：
--
作者：
関口 雄介;Yoshida Masayuki
与鱼的排泄运动有关的生物电信号可以通过外部电极检测到。在这项研究中，一种技术，连续监测在自由游泳斑马鱼的电刺激信号。该技术被应用于监测通气活动作为一种生理措施，结合各种行为措施，在一个新的坦克环境。结果发现，除了通风率，时域分析的通风率的变化是有用的评估情绪状态的斑马鱼。通过整合生理和行为的分析措施，1小时的新的坦克试验表明，习惯化过程包括两个阶段。第一阶段，持续10分钟，涉及快速衰减的最初的恐惧/焦虑反应，遇到一个新的环境。第二阶段持续20分钟，涉及进一步减弱焦虑和增加探索行为。这些数据表明，结合通风相关的生理措施与传统的行为措施，使多维检查的习惯化过程中的一个新的坦克环境，更精确的比可能时，仅依赖于行为反应。
Bioelectric signals related to ventilatory movements in fish can be detected via externally located electrodes. In this study, a technique to continuously monitor the electric ventilatory signals in free-swimming zebrafish was developed. This technique was applied to monitoring ventilation activity as a physiological measure in conjunction with various behavioral measures in a novel tank environment. It was found that in addition to ventilation rate, time domain analysis of changes in ventilation rate is useful for evaluating the emotional state of zebrafish. By integrating the physiological and behavioral measures in analyses, a 1 h novel tank test trial revealed that the habituation process involves two phases. The first phase, which lasted 10 min, involved rapid attenuation of the initial fear/anxiety response to encountering a novel environment. The second phase lasted 20 min and involved further attenuation of anxiety and an increase in exploration behavior. These data suggest that combining ventilation-related physiological measures with conventional behavioral measures enables multidimensional examination of the habituation process in a novel tank environment with more precision than is possible when relying on behavioral responses alone.