Role of anterior hypothalamic natriuretic peptide in lipopolysaccharide-induced fever in rats.
Role of anterior hypothalamic natriuretic peptide in lipopolysaccharide-induced fever in rats.
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下丘脑前部钠尿肽在脂多糖诱导的大鼠发热中的作用。
DOI：
10.1007/s00421-009-1157-8
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发表时间：
2010
影响因子：
3
通讯作者：
T.
中科院分区：
文献类型：
--
作者：
Miyoshi;M. and Watanabe;T.
我们调查是否在视前区，利钠肽（NP）作为一种内源性解热剂在大鼠全身给予细菌内毒素（脂多糖，LPS）发热。静脉注射（i. v.）注射LPS（2 μg/kg）引起三相热。这种发热的第三阶段（a）通过视前区内（i.p.o.）注射NP受体（A型和B型）拮抗剂HS-142-1（1 μg），（B）明显减弱（P < 0. 05）。注射心房NP（ANP，20 ng）。当静脉注射时，LPS诱导下丘脑前部C型NP的mRNA编码显著上调，并倾向于上调ANP。注射LPS的大鼠下丘脑前部IL-1β mRNA的表达显著高于注射生理盐水的大鼠。这些结果表明，静脉注射LPS后下丘脑前部内产生的NP可能作用于视前NP受体，以抑制LPS诱导的发热，可能是通过衰减LPS诱导的促炎细胞因子和/或随后的最终发热介质前列腺素E2的产生。
We investigated whether within the preoptic area, natriuretic peptide (NP) acts as an endogenous antipyretic in rats made febrile by systemic administration of bacterial endotoxin (lipopolysaccharide, LPS). Intravenous (i.v.) injection of LPS (2 μg/kg) induced a triphasic fever. The third phase of this fever was (a) significantly enhanced by an intrapreoptic (i.p.o.) injection of the NP-receptor (A-type and B-type) antagonist HS-142-1(1 μg), and (b) significantly attenuated by an i.p.o. injection of atrial NP (ANP, 20 ng). When given i.v., LPS induced significant upregulation of the mRNA coding for C-type NP within the anterior hypothalamus, and tended to upregulate that for ANP. The anterior hypothalamic expression of interleukin-1β mRNA was significantly greater in rats injected i.v. with LPS than in saline-injected rats. These results suggest that NPs produced within the anterior hypothalamus after i.v. injection of LPS may act upon preoptic NP receptors to inhibit the LPS-induced fever, possibly through attenuation of the LPS-induced production of proinflammatory cytokines and/or the subsequent final fever mediator prostaglandin E2.