The murine AE4 promoter predominantly drives type B intercalated cell specific transcription
The murine AE4 promoter predominantly drives type B intercalated cell specific transcription
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DOI：
10.1007/s00418-009-0614-0
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发表时间：
2009-10-01
影响因子：
2.3
通讯作者：
Kurth, Ingo
中科院分区：
文献类型：
--
作者：
Hentschke, Moritz;Hentschke, Suna;Kurth, Ingo
AE4 是一种阴离子交换剂，几乎只在肾集合管中表达。这种非常受限的表达促使我们更详细地分析其转录。 5' RACE 产生了替代转录起始位点，预计可编码 N 端蛋白质变体。比较物种之间的 5' 基因组序列，鉴定出具有三个保守跨度的转录活性区域。在转基因小鼠中，由该片段驱动的β-半乳糖苷酶表达类似于内源性AE4表达，并且主要限于B型嵌入细胞。因此，该启动子可能被证明可用于通过遗传方法靶向 B 型嵌入细胞。
AE4 is an anion exchanger almost exclusively expressed in the collecting ducts of the kidney. This very restricted expression prompted us to analyze its transcription in more detail. 5' RACE yielded alternative transcriptional start sites that are predicted to code for N-terminal protein variants. Comparison of the 5' genomic sequence between species identified a transcriptionally active region with three conserved spans. In transgenic mice beta-galactosidase expression driven by this fragment resembled endogenous AE4 expression and was predominantly restricted to type B intercalated cells. Hence this promoter could prove useful to target type B intercalated cells by genetic approaches.