Effect of correlation between traction forces on tensional homeostasis in clusters of endothelial cells and fibroblasts
Effect of correlation between traction forces on tensional homeostasis in clusters of endothelial cells and fibroblasts
复制标题
牵引力对内皮细胞和成纤维细胞簇张力稳态的影响
DOI：
10.1016/j.jbiomech.2019.109588
复制
发表时间：
2020
影响因子：
2.4
通讯作者：
Stamenovic, D
中科院分区：
文献类型：
--
作者：
Barbone, PE;Smith, ML;Stamenovic, D
细胞对外部和内部干扰保持恒定水平的细胞骨架张力的能力被称为张力稳态。它对于细胞和组织的正常生理功能以及防止疾病进展（包括动脉粥样硬化和癌症）至关重要。在以前的研究中，我们将张力稳态定义为细胞在低时间波动下维持细胞骨架张力一致水平的能力。在这些研究中，我们测量了内皮细胞和成纤维细胞簇中细胞基质牵引力的时间波动。我们观察到这些时间波动减少，在内皮细胞中，但不是在成纤维细胞中的集群大小的增加。我们通过牵引场的变异系数（CV）量化了时间波动，从而量化了动态平衡; CV值越低，细胞越接近张力动态平衡状态。该度量取决于各个牵引力之间的相关性。在这项研究中，我们分析了牵引力之间的相关性的贡献牵引场CV在集群的内皮细胞和成纤维细胞，使用我们以前获得的实验数据。我们的分析结果表明，牵引力之间的正相关性是有害的稳态，它是细胞类型依赖性。
The ability of cells to maintain a constant level of cytoskeletal tension in response to external and internal disturbances is referred to as tensional homeostasis. It is essential for the normal physiological function of cells and tissues, and for protection against disease progression, including atherosclerosis and cancer. In previous studies, we defined tensional homeostasis as the ability of cells to maintain a consistent level of cytoskeletal tension with low temporal fluctuations. In those studies, we measured temporal fluctuations of cell-substrate traction forces in clusters of endothelial cells and of fibroblasts. We observed those temporal fluctuations to decrease with increasing cluster size in endothelial cells, but not in fibroblasts. We quantified temporal fluctuation, and thus homeostasis, through the coefficient of variation (CV) of the traction field; the lower the value ofCV, the closer the cell is to the state of tensional homeostasis. This metric depends on correlation between individual traction forces. In this study, we analyzed the contribution of correlation between traction forces on traction fieldCVin clusters of endothelial cells and fibroblasts using experimental data that we had obtained previously. Results of our analysis showed that positive correlation between traction forces was detrimental to homeostasis, and that it was cell type-dependent.