Phylogenetic relationships and pathogenicity variation of two Newcastle disease viruses isolated from domestic ducks in Southern China
Phylogenetic relationships and pathogenicity variation of two Newcastle disease viruses isolated from domestic ducks in Southern China
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华南地区家鸭两种新城疫病毒的系统发育关系及致病性变异
DOI：
10.1186/1743-422x-11-147
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发表时间：
2014-08-12
期刊：
影响因子：
4.8
通讯作者：
Ren, Tao
中科院分区：
文献类型：
--
作者：
Kang, Yinfeng;Li, Yanling;Ren, Tao
新城疫(ND)是由禽副粘病毒1型(APMV-1)强毒株引起的一种被世界动物卫生组织列入世界动物卫生组织(OIE)名录的疾病，是一种地方性传染病，给养禽业造成了巨大的经济损失。新城疫病毒基因VII型和基因IX型是中国流行的优势基因，可能是近年来鸡群暴发疫情的原因之一。方法对2010年从中国南部地区分离的新城疫新城疫强毒SS-10和NH-10的全基因组序列、临床病理特征和传播情况进行了研究。结果F和基于系统发育树的全基因序列分析表明，SS-10(基因VII型)和NH-10(基因IX型)属于II类毒株，推测其融合蛋白切割位点的氨基酸序列为112R-R-Q-K/R-R-F(117)。动物实验结果表明，从鸭源分离的SS-10病毒对鸡、鹅具有较强的致病性，而对鸭的致病性较低。可从脾、肺、肾、气管、小肠、法氏囊、胸腺、胰腺和盲肠扁桃体、口咽和泄殖腔拭子中检出，并可传播给幼鸟。此外，它还可以通过幼稚的接触传播给鸡、鸭和鹅。然而，从鸭身上分离到的NH-10病毒可以感染一些鸡、鸭和鹅，但只会导致鸡的死亡。结论两株新城疫病毒分离株在鸡、鸭、鹅体内的致病性和传播性不同。因此，鸡、鸭、鹅三种新城疫病毒之间不存在种间偏好，应重视新城疫病毒在鸭、鹅、鸡之间的跨种传播，以控制和消灭新城疫。
BackgroundNewcastle disease (ND) is an OIE listed disease caused by virulent avian paramyxovirus type 1 (APMV-1) strains, which is enzootic and causes large economic losses in the poultry sector. Genotype VII and genotype IX NDV viruses were the predominant circulating genotype in China, which may possibly be responsible for disease outbreaks in chicken flocks in recent years. While ducks and geese usually have exhibited inapparent infections.MethodsIn the present study, we investigate the complete genome sequence, the clinicopathological characterization and transmission of two virulent Newcastle disease viruses, SS-10 and NH-10, isolated from domestic ducks in Southern China in 2010.ResultsF, and the complete gene sequences based on phylogenetic analysis demonstrated that SS-10 (genotype VII) and NH-10 (genotype IX) belongs to class II. The deduced amino acid sequence was(112)R-R-Q-K/R-R-F(117)at the fusion protein cleavage site. Animal experiment results showed that the SS-10 virus isolated from ducks was highly pathogenic for chickens and geese, but low pathogenic for ducks. It could be detected from spleen, lung, kidney, trachea, small intestine, bursa of fabricius, thymus, pancreas and cecal tonsils, oropharyngeal and cloacal swabs, and could transmit to the naive contact birds. Moreover, it could transmit to chickens, ducks and geese by naive contact. However, the NH-10 virus isolated from ducks could infect some chickens, ducks and geese, but only caused chickens to die. Additionally, it could transmit to the naive contact chickens, ducks, and geese.ConclusionThe two NDV isolates exhibited different biological properties with respect to pathogenicity and transmission in chickens, ducks and geese. Therefore, no species-preference exists for chicken, duck or goose viruses and more attention should be paid to the trans-species transmission of VII NDVs between ducks, geese and chickens for the control and eradication of ND.