Method for detecting the 3-hydroxymyristic acid component of the endotoxins of gram-negative bacteria in compost samples.
Method for detecting the 3-hydroxymyristic acid component of the endotoxins of gram-negative bacteria in compost samples.
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堆肥样品中革兰氏阴性菌内毒素3-羟基肉豆蔻酸成分的检测方法。
DOI：
10.1080/15298668591395661
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
Clark,CS
中科院分区：
文献类型：
--
作者：
Kirschner,D;QueHee,SS;Clark,CS
建立了大肠杆菌脂多糖（LPS）内毒素3-或β-羟基肉豆蔻酸（BHM）组分的化学分析方法。coli，完整的E.大肠杆菌和污水污泥堆肥。内毒素是与许多革兰氏阴性菌（如大肠杆菌）的外膜相关的热原。大肠杆菌和沙门氏菌。BHM酸含量用作内毒素存在的化学标志物，因为BHM酸存在于负责毒性的分子亚基（脂质-A）中，并且是该亚基中最丰富的饱和脂肪酸。因此，BHM酸定量补充了鲎试剂生物测定法，以检测棉花和其他灰尘、血液、水、堆肥和空气样品中存在的革兰氏阴性细菌。BHM酸经消化、乙醚萃取、碱解和乙醚萃取后分离。使用两个GC色谱柱作为筛选方法或通过GC/ MS用于确证目的，将游离酸定量为甲酯。回收率低于15 ng的BHM酸是不可靠的。用双柱筛选法测定了E. coliLPS，完整E.大肠杆菌和堆肥以μg BHM酸/ g基质计的平均值分别为（算术平均值±标准差）：56 120 ± 2200; 2650 ± 339;和14.7 ± 5.7。
An analytical chemical method for the 3- or beta-hydroxymyristic (BHM) acid component of endotoxins has been developed for the lipopolysaccharide (LPS) ofE. coli, intactE. coli, and sewage sludge compost. Endotoxins are the pyrogens associated with the outer membranes of many gram-negative bacteria, for example,E. coliandSalmonella. The BHM acid content was used as a chemical marker for endotoxin presence, since BHM acid is present in the molecular subunit (Lipid-A) responsible for the toxicity and is the most abundant saturated fatty acid in that subunit. BHM acid quantification thus complements theLimulusbioassay to detect gram-negative bacteria presence in such samples as cotton and other dusts, blood, water, compost and air samples. BHM acid was isolated after digestion, ether extraction, alkaline hydrolysis, and ether extraction. The free acid was quantitated as the methyl ester using two GC columns as a screening method or by GC/ MS for confirmatory purposes. Recoveries were unreliable below 15 ng of BHM acid. By the use of the 2-column screening technique, the amounts of BHM equivalent inE. coliLPS, intactE. coliand compost in μg BHM acid/ g substrate were (arithmetic mean ± standard deviation), respectively: 56 120 ± 2200; 2650 ± 339; and 14.7 ± 5.7.