Isolation and Phenotypic Characterization of Cancer Stem Like Cells from Nasopharyngeal Carcinoma
Isolation and Phenotypic Characterization of Cancer Stem Like Cells from Nasopharyngeal Carcinoma
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DOI：
10.1055/s-0034-1382077
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发表时间：
2015-06-01
期刊：
影响因子：
2.2
通讯作者：
Li, S. -L.
中科院分区：
文献类型：
--
作者：
Wang, Y.;Zhang, G. -H.;Li, S. -L.
前言：最近的研究报告，癌症干细胞（CSC）是治疗失败和肿瘤复发的原因。CSC已经在几种实体瘤中分离和表征。方法：采用Hoechst 33342染料排斥法，采用流式细胞仪（FACS）对鼻咽癌组织中的SP样癌干细胞进行鉴定，并对SP样癌干细胞的形态、分化流式细胞仪分选后的SP细胞进一步进行耐药实验、克隆形成实验和免疫细胞化学分析CD 44和ABCG 2的表达。结果：从鼻咽癌中鉴定出3.9%的肿瘤干细胞样SP细胞。经维拉帕米处理后，SP细胞的百分比降低至0.9%。流式细胞仪分选的SP细胞显示出CD 44和ABCG 2的表达增强。此外，这些SP细胞是高度耐多药和高效力的球体形成时相比，non-SP cells.Conclusions：我们的数据清楚地表明，存在的癌症干细胞样SP细胞从鼻咽癌可能负责化疗药物耐药，肿瘤复发和侵袭。
Introduction: Recent studies reported that cancer stem cells (CSCs) are responsible for treatment failure and tumor recurrence. CSCs have been isolated and characterized in several solid tumors. However, the information regarding the CSCs from nasopharyngeal carcinoma (NPC) is very less. Therefore, in the current study we aimed to isolate and characterize the cancer stem like SP cells from NPC.Methods: We have used FACS based Hoechst 33342 dye exclusion technique to identify the cancer stem like SP cells from NPC. The FACS sorted SP cells are further subjected to drug resistance assay, clonogenic formation assay and immunocytochemistry analysis for the expression of CD44 and ABCG2.Results: We have identified 3.9% of cancer stem like SP cells from NPC. Upon treatment with verapamil, the percentage of SP cells was reduced to 0.9%. The FACS sorted SP cells showed enhanced expression of CD44 and ABCG2. Further, these SP cells are highly resistant to multi-drugs and high potent of sphere formation when compared to the non-SP cells.Conclusions: Our data clearly suggest that presence of cancer stem like SP cells from nasopharyngeal carcinoma might responsible for chemotherapeutic drug resistance, tumor recurrence and invasion.