Changes in the abundance of polyadenylated RNAs in development of Drosophila melanogaster analyzed with cloned DNA fragments.
Changes in the abundance of polyadenylated RNAs in development of Drosophila melanogaster analyzed with cloned DNA fragments.
复制标题
用克隆 DNA 片段分析果蝇发育过程中聚腺苷酸化 RNA 丰度的变化。
DOI：
10.1016/s0012-1606(81)80008-5
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发表时间：
1981
影响因子：
2.7
通讯作者：
H. Biessmann
中科院分区：
文献类型：
--
作者：
H. Biessmann
通过在硝酸纤维素滤膜上与克隆的双链cDNA片段杂交，测定单个细胞质poly（A）RNA的稳态浓度。适当控制DNA过量杂交反应和X射线胶片曝光条件，以进行放射自显影的定量分析。对黑腹果蝇Kc组织培养细胞、胚胎、三龄幼虫、蛹和成虫的mRNA群体进行了分析。我们的研究结果表明，在发展的不同阶段表现出不同的mRNA群体相对于本研究中采用的20个基因。这是由于单个mRNA在发育过程中表现出特征性变化，反映了mRNA复杂性或丰度的波动。
Steady-state concentrations of individual cytoplasmic poly (A) RNAs were determined by hybridization to cloned double-stranded cDNA fragments on nitrocellulose filters. The DNA excess hybridization reaction and X-ray film exposure conditions were properly controlled to allow quantitative analysis of the autoradiograms. mRNA populations from Kc tissue culture cells, embryos, third-instar larvae, pupae, and adults ofDrosophila melanogasterwere analyzed. Our results show that different stages in development exhibit distinct mRNA populations with respect to 20 genes employed in this study. This is due to individual mRNAs which showed characteristic changes during development reflecting the fluctuations in mRNA complexity or abundance.