Phosphorylation of histone H4 Ser1 regulates sporulation in yeast and is conserved in fly and mouse spermatogenesis
Phosphorylation of histone H4 Ser1 regulates sporulation in yeast and is conserved in fly and mouse spermatogenesis
复制标题
DOI：
10.1101/gad.1457006
复制
发表时间：
2006-09-15
影响因子：
10.5
通讯作者：
Berger, Shelley L.
中科院分区：
文献类型：
--
作者：
Krishnamoorthy, Thanuja;Chen, Xin;Berger, Shelley L.
酿酒酵母中的孢子形成是一个高度调控的过程，其中二倍体细胞产生四个单倍体配子。在这项研究中，我们表明，组蛋白H4丝氨酸1磷酸化（H4 S1 ph）在孢子形成过程中，从中期孢子形成和持续到萌发，并在时间上不同于早期减数分裂连锁H3 S10 ph参与染色体凝聚。组蛋白H4 S1 A置换突变体形成异常孢子并具有降低的孢子形成效率。删除孢子形成特异性酵母Sps 1，一个成员的Ste 20家族的激酶，几乎废除了孢子形成相关的H4 S1 ph修改。H4 S1 ph可以促进染色质致密化，因为缺失SPS 1增加了抗体免疫沉淀的可及性;此外，缺失SPS 1或H4 S1 A取代导致孢子内细胞核中DNA体积增加。我们发现H4 S1 ph存在于果蝇和小鼠精子发生过程中，与酵母相似，这种修饰相对于H3 S10 ph延伸到精子分化晚期。因此，H4 S1 ph可能是一种进化上古老的组蛋白修饰，用于标记配子相关包装的基因组。
Sporulation in Saccharomyces cerevisiae is a highly regulated process wherein a diploid cell gives rise to four haploid gametes. In this study we show that histone H4 Ser1 is phosphorylated ( H4 S1ph) during sporulation, starting from mid-sporulation and persisting to germination, and is temporally distinct from earlier meiosis-linked H3 S10ph involved in chromosome condensation. A histone H4 S1A substitution mutant forms aberrant spores and has reduced sporulation efficiency. Deletion of sporulation-specific yeast Sps1, a member of the Ste20 family of kinases, nearly abolishes the sporulation-associated H4 S1ph modification. H4 S1ph may promote chromatin compaction, since deletion of SPS1 increases accessibility to antibody immunoprecipitation; furthermore, either deletion of Sps1 or an H4 S1A substitution results in increased DNA volume in nuclei within spores. We find H4 S1ph present during Drosophila melanogaster and mouse spermatogenesis, and similar to yeast, this modification extends late into sperm differentiation relative to H3 S10ph. Thus, H4 S1ph may be an evolutionarily ancient histone modification to mark the genome for gamete-associated packaging.