Alkaline denaturation of dentin--A simple way to isolate human tooth enamel for electron spin resonance dosimetry.
Alkaline denaturation of dentin--A simple way to isolate human tooth enamel for electron spin resonance dosimetry.
复制标题
牙本质的碱变性——一种分离人牙釉质用于电子自旋共振剂量测定的简单方法。
DOI：
10.1269/jrr.38.173
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发表时间：
1997
影响因子：
2
通讯作者：
C. Miyazawa
中科院分区：
文献类型：
--
作者：
N. Nakamura;C. Miyazawa
牙釉质电子自旋共振（ESR）是最近开发的一种用于回顾性估计人体辐射剂量的方法。该测定需要将牙釉质与牙本质分离，这很困难，因为牙釉质和牙本质之间的边界不易辨别。在这里，我们描述了一种通过牙本质碱变性分离牙釉质的简单方法。该方法需要 4 周，但每周只需刮擦变性并软化的牙本质一次。最重要的是，不需要特殊技能。我们发现，碱性处理不会导致在隔离之前暴露于 2 Gy 伽马射线的牙釉质中记录的 ESR 信号恶化。该测定特别适用于拔牙后长期储存期间产生的含有许多裂纹的牙齿。这种牙齿在牙釉质分离过程中往往会崩解，这给以机械方式将牙釉质与单个小碎片分离带来了困难。
Electron spin resonance (ESR) of tooth enamel is a recently developed method for the retrospective dose estimation of human radiation exposures. The assay requires isolation of enamel from dentin, which is difficult because the boundary between enamel and dentin is not easily discernible. Here we describe a simple method for isolating enamel by alkaline denaturation of dentin. The method requires 4 weeks, but scratching of the denatured and hence softened dentin is needed only once a week. Above all, no special skill is required. We found that the alkaline treatment did not cause deterioration of the ESR signal recorded in enamel exposed to 2 Gy of gamma rays prior to its isolation. The assay is particularly suited for teeth containing many cracks that were generated during long-term storage after extraction of the teeth. Such teeth tend to disintegrate during enamel isolation processes, which poses difficulties to isolate enamel mechanically from individual small pieces.