DEVELOPMENT OF A SIMPLE INDIRECT ENZYME-LINKED-IMMUNOSORBENT-ASSAY FOR THE DETECTION OF IMMUNOGLOBULIN-M ANTIBODY IN SERUM FROM PATIENTS FOLLOWING AN OUTBREAK OF CHIKUNGUNYA VIRUS-INFECTION IN YANGON, MYANMAR
DEVELOPMENT OF A SIMPLE INDIRECT ENZYME-LINKED-IMMUNOSORBENT-ASSAY FOR THE DETECTION OF IMMUNOGLOBULIN-M ANTIBODY IN SERUM FROM PATIENTS FOLLOWING AN OUTBREAK OF CHIKUNGUNYA VIRUS-INFECTION IN YANGON, MYANMAR
复制标题
DOI：
10.1016/0035-9203(92)90260-j
复制
发表时间：
1992-07-01
影响因子：
2.2
通讯作者：
MYINT, A
中科院分区：
文献类型：
--
作者：
THEIN, S;LALINN, M;MYINT, A
1984年，缅甸仰光儿童医院收治了1548名患有出血热的儿童。在获得配对血清的803名儿童中，有577名没有发现近期感染登革热的证据，这增加了缅甸再次出现基孔肯雅病毒感染的可能性。仅使用市售试剂或无需使用精密设备即可制备的试剂来制备和标准化用于检测抗基孔肯雅病毒免疫球蛋白M（IgM）抗体的酶联免疫吸附测定（ELISA）。虽然血凝抑制 (HI) 测试和 IgM ELISA 在诊断急性基孔肯雅病毒感染方面有 90% 的一致性，但使用 ELISA 可以将另外 12 名具有固定抗基孔肯雅病毒 HI 抗体滴度的患者识别为患有急性基孔肯雅病毒感染。此外，入院时 ELISA 可以识别的患者数量 (31/103) 是 HI 测试 (15/103) 的两倍。 ELISA 没有出现假阳性 IgM 反应，这可能是由于类风湿因子的存在所致。通过该测试，在仰光儿童医院就诊的163名发烧/出血热儿童样本中，有103名被诊断为基孔肯雅热患者，4名可能患有基孔肯雅热和登革热双重感染，16名患有登革热，30名既没有基孔肯雅热也没有登革热感染，还有10名患者无法做出明确诊断。常规使用 ELISA 可以提醒当局未来基孔肯雅病毒感染的爆发，并避免将患有不危及生命的病毒性疾病的患者收治入院。
During 1984, 1548 children were admitted to the Yangon [Rangoon] Children's Hospital in Myanmar [Burma] with haemorrhagic fever. No evidence of recent dengue infection was found in 577 of the 803 children from whom paired sera were obtained, raising the possibility of reappearance of Chikungunya virus infection in Myanmar. An enzyme-linked immunosorbent assay (ELISA) for the detection of anti-Chikungunya virus immunoglobulin M (IgM) antibody was prepared and standardized using only reagents which are commercially available or which could be prepared without the use of sophisticated equipment. While there was 90% agreement between haemagglutination inhibition (HI) tests and the IgM ELISA in the diagnosis of acute Chikungunya virus infections, 12 additional patients with stationary anti-Chikungunya virus HI antibody titres could be identified as having acute Chikungunya infections using the ELISA. Furthermore, the ELISA could identify twice as many patients (31/103) at the time of admission to hospital as the HI test (15/103). There was no false positive IgM reaction with the ELISA WhiCh could be attributed to the presence of rheumatoid factor. Using the test, 103 of a sample of 163 children who presented to the Yangon Children's Hospital with fever/haemorrhagic fever were diagnosed as Chikungunya patients, 4 had possible dual Chikungunya and dengue infections, 16 had dengue, 30 had neither Chikungunya nor dengue infections, and a definitive diagnosis could not be made for 10 patients. Routine use of the ELISA would alert authorities to future outbreaks of Chikungunya virus infection and avoid admission to hospital of patients with a non-life-threatening viral disease.