Rapid identification of Escherichia coli safety and laboratory strain lineages based on Multiplex-PCR
Rapid identification of Escherichia coli safety and laboratory strain lineages based on Multiplex-PCR
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DOI：
10.1111/j.1574-6968.2006.00594.x
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发表时间：
2007-04-01
影响因子：
2.1
通讯作者：
Schleifer, Karl-Heinz
中科院分区：
文献类型：
--
作者：
Bauer, Andreas Peter;Dieckmann, Sarah Maria;Schleifer, Karl-Heinz
大肠杆菌K-12、B、C和W菌株是最常用的细菌安全和实验室菌株。通过微板消减杂交检测谱系特异性DNA片段，并利用该片段创建使用单个PCR反应的快速区分方法，以清楚地区分四个谱系并将它们与致病性变体分离。该方法已在广泛使用的实验室菌株和多种致病性大肠杆菌的综合选择进行了评价。大肠杆菌代表。此外，对所有可用的E.大肠杆菌基因组和近亲志贺氏菌和沙门氏菌的基因组证实了所提出的方法的可靠性。快速鉴定和鉴别E.多重PCR检测大肠杆菌安全菌株是研究人员和公司检查和监测其参考库存的有用工具。
Escherichia coli K-12, B, C and W strains are the most frequently used bacterial safety and laboratory strains. Lineage-specific DNA fragments were detected by microplate subtractive hybridization and utilized to create a fast differentiation method using a single PCR reaction to differentiate clearly the four lineages and separate them from pathogenic variants. The method has been evaluated on a comprehensive selection of widely used laboratory strains and a variety of pathogenic E. coli representatives. In addition, in silico analysis on all available E. coli genomes and the genomes of the close relatives Shigella and Salmonella confirmed the reliability of the proposed method. A fast identification and differentiation of E. coli safety strains by Multiplex-PCR is a useful tool for researchers and companies to check and monitor their reference stocks.