Identification of Proteins Associated with Polyhydroxybutyrate Granules from Herbaspirillum seropedicae SmR1 - Old Partners, New Players
Identification of Proteins Associated with Polyhydroxybutyrate Granules from Herbaspirillum seropedicae SmR1 - Old Partners, New Players
复制标题
与 Herbaspirillum seropedicae SmR1 中的聚羟基丁酸酯颗粒相关的蛋白质的鉴定 - 老伙伴，新玩家
作者：
Evandro F. Tirapelle;M. Müller;M. Tadra;M. Kadowaki;M. Steffens;R. Monteiro;E. M. Souza;F. Pedrosa;L. S. Chubatsu
Seropedicae草杆菌是一种与重要农作物相关的重氮菌。这种细菌产生聚羟基丁酸酯(PHB)，一种脂肪族聚酯，作为碳储存和/或还原当量的来源。PHB聚合物主要以包被蛋白质的细胞内不溶颗粒的形式储存，其中一些蛋白质直接参与PHB的合成、降解和颗粒的生物发生。在这项工作中，我们从血清中提取了PHB颗粒，并用质谱仪鉴定了它们的相关蛋白。这一分析使我们能够确定包被PHB颗粒的主要Phasin(PhaP1)以及负责其合成的PHB合成酶(PhbC1)。一个phbC1突变体在PHB的合成中受到损害，证实了它在H.seropedicae中的作用。另一方面，phaP1突变体产生PHB颗粒，但主要包被次级Phasin(PhaP2)。此外，还鉴定了一些以前未被描述的与PHB代谢有关的新蛋白，这为PHB在血清医学中的功能带来了新的可能性。
Herbaspirillum seropedicae is a diazotrophic ß-Proteobacterium found associated with important agricultural crops. This bacterium produces polyhydroxybutyrate (PHB), an aliphatic polyester, as a carbon storage and/or source of reducing equivalents. The PHB polymer is stored as intracellular insoluble granules coated mainly with proteins, some of which are directly involved in PHB synthesis, degradation and granule biogenesis. In this work, we have extracted the PHB granules from H. seropedicae and identified their associated-proteins by mass spectrometry. This analysis allowed us to identify the main phasin (PhaP1) coating the PHB granule as well as the PHB synthase (PhbC1) responsible for its synthesis. A phbC1 mutant is impaired in PHB synthesis, confirming its role in H. seropedicae. On the other hand, a phaP1 mutant produces PHB granules but coated mainly with the secondary phasin (PhaP2). Furthermore, some novel proteins not previously described to be involved with PHB metabolism were also identified, bringing new possibilities to PHB function in H. seropedicae.
影响因子：
3.2
作者：
Pfeiffer, Daniel;Jendrossek, Dieter
通讯作者：
Jendrossek, Dieter