Oxygen taxis and proton motive force in Salmonella typhimurium.
Oxygen taxis and proton motive force in Salmonella typhimurium.
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发表时间：
1984-09
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通讯作者：
J. Shioi;B. L. Taylor
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作者：
J. Shioi;B. L. Taylor
鼠伤寒沙门氏菌SL 3730的趋氧性反应与通过流动透析技术测量的质子动力（Δ p）的变化定量相关。在pH7.5时，S.当使需氧生长的细胞厌氧时，鼠伤寒沙门氏菌的电压从-162 +/-13变为-111 +/-15 mV，当细胞恢复需氧时，电压恢复到原始值。跨膜的Δ pH为零。在pH 5.5时，Δ psi在好氧生活中为-70 mV，在厌氧生活中为-20 mV，好氧和厌氧细胞的Δ pH分别为-118和-56 mV。Δ P的减少导致翻滚增加，而Δ P的增加导致在任一pH值下的平滑游泳反应。在pH 7.5下由各种浓度的KCN抑制嗜氧性与Δ P减少相关，这是由于好氧生活中Δ psi减少，而在厌氧生活中Δ psi变化不大。在浓度高达100 mM时，2，4-二硝基苯酚降低Δ psi，但不抑制嗜氧性，因为Δ psi在好氧和厌氧细胞之间的差异保持恒定。从整体上看，S.鼠伤寒沙门氏菌是由Δ P介导的。
The aerotactic response of Salmonella typhimurium SL3730 has been quantitatively correlated with a change in the proton motive force (delta p) as measured by a flow-dialysis technique. At pH 7.5, the membrane potential (delta psi) in S. typhimurium changed from -162 +/- 13 to -111 +/- 15 mV when cells grown aerobically were made anaerobic, and it returned to the original value when the cells were returned to aerobiosis. The delta pH across the membrane was zero. At pH 5.5, delta psi was -70 mV in aerobiosis and -20 mV in anaerobiosis, and delta pH was -118 and -56 mV for aerobic and anaerobic cells, respectively. A decrease in delta p resulted in increased tumbling, and an increase in delta p resulted in a smooth swimming response at either pH. Inhibition of aerotaxis at pH 7.5 by various concentrations of KCN correlated with a decreased delta p, due to a decreased delta psi in aerobiosis and little change in delta psi in anaerobiosis. At concentrations up to 100 mM, 2,4-dinitrophenol decreased delta psi, but did not inhibit aerotaxis because the difference between delta psi in aerobic and anaerobic cells remained constant. Considered as a whole, the results indicate that aerotaxis in S. typhimurium is mediated by delta p.