Immunocytochemical identification of primary olfactory afferents in rainbow trout
Immunocytochemical identification of primary olfactory afferents in rainbow trout
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虹鳟鱼初级嗅觉传入的免疫细胞化学鉴定
DOI：
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发表时间：
1992
期刊：
影响因子：
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通讯作者：
B. Oakley
中科院分区：
文献类型：
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作者：
D. Riddle;B. Oakley
我们已经使用了一个组合的技术来分析鳟鱼的初级嗅觉投射：顺行道跟踪辣根过氧化物酶（HRP）和免疫细胞化学与抗血清的嗅觉标记蛋白（OMP）和匙孔血蓝蛋白（KLH）。HRP标记和OMP抗血清揭示了一个纤毛受体神经元的子集，具有宽树突，缺乏在其他受体神经元上发现的突出旋钮。初级嗅觉轴突的组织清楚地揭示了KLH的抗血清，没有其他神经元的反应。当用抗KLH可视化时，嗅轴突束在分散的位点穿透嗅玫瑰花结的基底层并会聚形成嗅神经。嗅神经内的束平行于神经的长轴行进，直到它们进入嗅球时被广泛的混合物重新利用。在嗅球内，大多数轴突终止于肾小球层中的9个离散终末野;然而，少数嗅神经轴突投射到端脑腹内侧。供应每个终端领域在肾小球层的神经束遵循独特的轨迹内的嗅神经层。在两个终末区域终止的轴突形成刷状终止，而不是其余七个区域特有的肾小球终止。单侧嗅神经切断后，返回的嗅轴突在14周内重新建立了正常的终末野模式。这是可能的，在鳟鱼嗅球传入的组织同样受到良好的调节，在正常的受体细胞更换。
We have used a combination of techniques to analyze the primary olfactory projection in trout: anterograde tract tracing with horseradish peroxidase (HRP) and immunocytochemistry with antisera to olfactory marker protein (OMP) and to keyhole limpet hemocyanin (KLH). HRP labeling and the OMP antiserum revealed a subset of ciliated receptor neurons with a wide dendrite that lacked the protruding knob found on other receptor neurons. The organization of the primary olfactory axons was clearly revealed by antisera to KLH, which reacted with no other neurons. When visualized with anti‐KLH, fascicles of olfactory axons penetrated the basal lamina of the olfactory rosette at scattered sites and converged to form the olfactory nerve. Fascicles within the olfactory nerve traveled parallel to the long axis of the nerve until resorted by extensive intermixing as they entered the olfactory bulb. Within the olfactory bulb, most axons terminated in nine discrete terminal fields in the glomerular layer; however, a few olfactory nerve axons projected into the ventral medial telencephalon. Fascicles supplying each terminal field in the glomerular layer followed distinctive trajectories within the olfactory nerve layer. Axons ending in two terminal fields made brush‐like terminations rather than the glomerular terminations characteristic of the remaining seven fields. After unilateral olfactory nerve transection, returning olfactory axons reestablished the normal pattern of terminal fields within 14 weeks. It is likely that the organization of afferents in the trout olfactory bulb is similarly well regulated during normal receptor cell replacement.