NERVE GROWTH-FACTOR REGULATES EXPRESSION OF NEUROPEPTIDE GENES IN ADULT SENSORY NEURONS
NERVE GROWTH-FACTOR REGULATES EXPRESSION OF NEUROPEPTIDE GENES IN ADULT SENSORY NEURONS
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DOI：
10.1038/337362a0
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发表时间：
1989-01-26
期刊：
影响因子：
64.8
通讯作者：
HARMAR, AJ
中科院分区：
文献类型：
--
作者：
LINDSAY, RM;HARMAR, AJ
神经生长因子（NGF）是个体发育期间交感神经元和感觉神经元存活所必需的营养分子1。然而，NGF参与维持或调节成熟外周神经元分化表型的程度还不太清楚。由于缺乏对NGF有反应但不需要其存活的神经元细胞的制备物，阻碍了对NGF对外周神经元作用的生化分析。我们在这里报告，在成人背根神经节神经元，它可以被分离，富集和保持在文化中的神经生长因子2的情况下，表达的mRNA编码的前体的两种神经肽，P物质和降钙素基因相关肽的调节神经生长因子。我们的研究结果提供了第一个直接的证据，在成熟的感觉神经元中的肽类神经递质/神经调质水平的调节，神经生长因子的连续动态作用。
Nerve growth factor (NGF) is a trophic molecule essential for the survival of sympathetic and sensory neurons during ontogeny1. The extent to which NGF is involved in the maintenance or regulation of the differentiated phenotypes of mature peripheral neurons is much less clear, however. Biochemical analysis of the actions of NGF upon peripheral neurons has been hampered by the lack of a preparation of neuronal cells that are responsive to NGF but do not require it for survival. We report here that in adult dorsal root ganglion neurons, which can be isolated, enriched and maintained in culture in the absence of neuronal growth factors2, the expression of mRNAs encoding the precursors of two neuropeptides, substance P and calcitonin gene-related peptide is regulated by NGF. Our results provide the first direct evidence of a continuous dynamic role for NGF in regulation of peptide neurotransmitter/neuromodulator levels in mature sensory neurons.