Fetoplacental angiogenesis during gestation is biphasic, longitudinal and occurs by proliferation and remodelling of vascular endothelial cells
Fetoplacental angiogenesis during gestation is biphasic, longitudinal and occurs by proliferation and remodelling of vascular endothelial cells
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DOI：
10.1053/plac.2002.0865
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发表时间：
2002-11-01
期刊：
影响因子：
3.8
通讯作者：
Mayhew, TM
中科院分区：
文献类型：
--
作者：
Mayhew, TM
绒毛的发育和成熟依赖于胎儿胎盘血管生成，但缺乏关于潜在结构事件的详细基线定量数据。本研究的目的是利用不同妊娠期胎盘的显微切片分析绒毛和胎儿血管生长的时空模式。重点是获取绝对数据，而不是相对数据。在妊娠10-41周收集的随机组织样品进行体视学分析。在“茎”，“中间”和“终端”绒毛（根据绒毛直径和胎儿血管的性质分类）的生长策略进行了评估的总体积，长度和平均横截面积。总体积、总长度和相对长度、平均横截面积和形状、血管内皮细胞数量和平均细胞大小（鳞状表面积）用于检查毛细血管生长。“中间”和“末端”绒毛的伸长和变薄使绒毛的伸长和变薄显著增加。绒毛成熟涉及毛细血管的差异性生长，其线性生长而平均横截面积无变化。纵向生长是由于内皮细胞增殖和平均细胞面积增加。事件的特征是在妊娠中期出现一个共同的拐点。通常情况下，一个早期缓慢的增长阶段，其次是一个更快的增长，但C毛细管：绒毛长度比和毛细管形状因子往往在妊娠中期达到峰值。研究结果证实，血管生成与绒毛的生长和成熟有关，并且是双相的，在妊娠中期左右发生血管内容物和排列的显著变化。毛细血管生长优先发生，并且仅通过持续增殖和随后的内皮细胞重塑驱动的总长度增加来实现。虽然没有提供分支血管生成与非分支血管生成的证据，但这些观察结果与胎儿胎盘血管生成发生在两个阶段的范例一致，开关与子宫胎盘氧张力，局部生长因子受体及其配体的变化有关。胎盘（2002），23，742-750（C）2002 Elsc icr Science Ltd.保留所有权利。
Villous development and maturation depend on fetoplacental angiogenesis but detailed baseline quantitative data on underlying structural events are lacking. The present aim was to analyse temporospatial patterns of villous and fetal vascular growth using e microscopical sections of placentae at different periods of gestation. The emphasis is on acquiring absolute rather than relative data. Random tissue samples collected at 10-41 weeks of gestation were processed for stereological analyses. Growth strategies in 'stem', 'intermediate' and 'terminal' villi (classified according to villous diameter and nature of fetal vessels were evaluated in terms of total volumes, lengths and mean cross-sectional areas. Total volume, total and relative length, mean cross-sectional area and shape, vascular endothelial cell number and mean cell size (squame surface area) were used to examine capillary growth. Volumes of "intermediate' and 'terminal' villi increased significantly due to elongation and thinning. Villous maturation involved differential growth of capillaries which grew linearly without changes in mean cross-scctional area. Longitudinal growth was due to endothelial cell proliferation and increases in mean cell area. Events were characterized by a common inflection point at about mid-gestation. Usually, an early slow growth phase was followed by one of faster growth but C capillary : villus length ratios and capillary shape factors tended to peak at mid-gestation. Findings confirm that angiogenesis is linked to villus growth and maturation, and is biphasic with dramatic changes in vascular content and arrangement occurring around mid-gestation. Capillary growth occurs preferentially and is solely by increase in total length driven by continuous proliferation and, later, by endothelial cell remodelling. Though providing no evidence for branching versus non-branching angiogenesis, these observations are consistent with the paradigm that fetoplacental angiogenesis occurs in two phases, the switch being associated with changes in uteroplacental oxygen tensions, local growth factor receptors and their ligands. Placenta (2002), 23, 742-750 (C) 2002 Elsc icr Science Ltd. All rights reserved.