Carbonic anhydrase IX, MN/CA IX: Analysis of stomach complementary DNA sequence and expression in human and rat alimentary tracts
Carbonic anhydrase IX, MN/CA IX: Analysis of stomach complementary DNA sequence and expression in human and rat alimentary tracts
复制标题
DOI：
10.1053/gast.1997.v112.pm9024293
复制
发表时间：
1997-02-01
期刊：
影响因子：
29.4
通讯作者：
Pastorek, J
中科院分区：
文献类型：
--
作者：
Pastorekova, S;Parkkila, S;Pastorek, J
背景和目标：CA IX（以前称为MN蛋白）是一种碳酸酐酶同工酶，其表达与人类肿瘤有关，但在正常胃粘膜中也有发现。本研究的目的是确定互补DNA（cDNA）的差异，以获得消化道分布的概述，并获得肿瘤中表达的数据。将从人胃文库中分离的CA 9 cDNA与来自HeLa细胞的cDNA一起沿着测序，使用CA IX特异性单克隆抗体和抗人CA II的兔抗血清进行人和动物组织的Western印迹和免疫组织化学分析，结果如下：序列分析表明，胃和HeLa细胞来源的cDNA之间没有差异，CA IX在胃，肠，胆囊上皮细胞的基底面检测。结论：CA IX在正常组织和肿瘤组织中的差异表达与其cDNA突变无关，CA IX蛋白在脊椎动物中的进化保守性以及在正常人胃粘膜中的高表达，提示CA IX蛋白具有重要的生理意义。
Background & Aims: CA IX (formerly MN protein) is a carbonic anhydrase isoenzyme whose expression is associated with human tumors, However, it has also been found in normal gastric mucosa. The aim of this study was to determine differences in complementary DNAs (cDNAs), to obtain an overview of distribution in the alimentary tract, and to obtain data on expression in tumors, Methods: A CA9 cDNA isolated from a human stomach library was sequenced along with the cDNA derived from HeLa cells, Western blotting and immunohistochemical analyses of human and animal tissues were performed using CA IX-specific monoclonal antibody and rabbit antiserum to human CA II, Results: Sequence analysis showed no differences between the stomach- and HeLa-derived cDNAs, CA IX was detected at the basolateral surface of gastric, intestinal, and gallbladder epithelia. In stomach tumor samples, expression of CA IX was lost or reduced, Conclusions: Differential distribution of CA IX in normal and tumor tissues is not associated with cDNA mutations, Evolutionary conservation in vertebrates as well as abundant expression of CA IX protein in normal human gastric mucosa, but not in derived tumors, indicate its physiological importance.