Nanoporous gold as a neural interface coating: effects of topography, surface chemistry, and feature size.
Nanoporous gold as a neural interface coating: effects of topography, surface chemistry, and feature size.
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DOI：
10.1021/acsami.5b00410
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发表时间：
2015-04-08
影响因子：
9.5
通讯作者：
Seker E
中科院分区：
文献类型：
--
作者：
Chapman CA;Chen H;Stamou M;Biener J;Biener MM;Lein PJ;Seker E
设计保持神经元与电极表面的紧密物理耦合的神经电极接口仍然是植入式和体外神经记录电极阵列的主要挑战。通常，低阻抗纳米结构电极涂层依赖于来自药物或表面固定肽的化学线索来抑制电极表面上的神经胶质瘢痕组织形成（星形胶质细胞增生），这是可靠的神经元-电极耦合的障碍。纳米多孔金（np-Au），由合金腐蚀过程中产生的，是一个很有前途的候选人，以减少星形胶质细胞增生只通过地形利用其可调的长度尺度。在目前的体外研究的np-Au的相互作用与皮质神经元-神经胶质细胞共培养物，我们证明了np-Au的纳米结构是通过保持高的神经元-星形胶质细胞表面覆盖率实现神经元的密切的物理耦合。原子层沉积为基础的表面改性，解耦的表面化学形态的影响。此外，长度尺度效应进行了系统的研究，通过控制的特征尺寸的np-Au的脱合金条件的变化。我们的研究结果表明，np-Au纳米形貌，而不是表面化学，减少星形胶质细胞表面覆盖，同时保持高神经元覆盖，并可能通过纳米结构介导的抑制瘢痕组织形成增强神经元-电极耦合。
Designing neural-electrode interfaces that maintain close physical coupling of neurons to the electrode surface remains a major challenge for both implantable and in vitro neural recording electrode arrays. Typically, low-impedance nanostructured electrode coatings rely on chemical cues from pharmaceuticals or surface-immobilized peptides to suppress glial scar tissue formation over the electrode surface (astrogliosis), which is an obstacle to reliable neuron-electrode coupling. Nanoporous gold (np-Au), produced by an alloy corrosion process, is a promising candidate to reduce astrogliosis solely through topography by taking advantage of its tunable length scale. In the present in vitro study on np-Au’s interaction with cortical neuron-glia co-cultures, we demonstrate that the nanostructure of np-Au is achieving close physical coupling of neurons through maintaining a high neuron-to-astrocyte surface coverage ratio. Atomic layer deposition-based surface modification was employed to decouple the effect of morphology from surface chemistry. Additionally, length scale effects were systematically studied by controlling the characteristic feature size of np-Au through variations of the dealloying conditions. Our results show that np-Au nanotopography, not surface chemistry, reduces astrocyte surface coverage while maintaining high neuronal coverage, and may enhance the neuron-electrode coupling through nanostructure-mediated suppression of scar tissue formation.