A TEMPERATURE SENSITIVE MUTANT OF SACCHAROMYCES-CEREVISIAE DEFECTIVE IN PRE-RIBOSOMAL-RNA PROCESSING
A TEMPERATURE SENSITIVE MUTANT OF SACCHAROMYCES-CEREVISIAE DEFECTIVE IN PRE-RIBOSOMAL-RNA PROCESSING
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DOI：
10.1093/nar/19.18.5059
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发表时间：
1991-09-25
影响因子：
14.9
通讯作者：
WARNER, JR
中科院分区：
文献类型：
--
作者：
SHUAI, K;WARNER, JR
一个隐性温度敏感突变体已经被分离出来，在核糖体RNA加工中有缺陷。Northern分析发现，该突变体积累了三个新的rRNA物种：23S‘, 18S’和7S'，每个物种都包含25S和18S rRNA之间的间隔区序列。35S pre-rRNA积累，而20S和27S rRNA加工中间体水平下降。脉冲追踪分析表明35S pre-rRNA的处理速度减慢。突变体的缺陷似乎出现在第一个加工步骤，即产生20S和27S rRNA。7S‘ RNA是5.8S RNA的一种形式，其5’端延长了149个核苷酸，位于正常裂解位点下游仅5个核苷酸的位置，产生20S和27S rRNA。7S’RNA可以组装成60S核糖体亚基，但这些亚基在连接多核糖体时相对无效。单个损伤是导致前rrna加工缺陷和温度敏感性的原因。受影响的基因被命名为RRP2。
A recessive temperature sensitive mutant has been isolated that is defective in ribosomal RNA processing. By Northern analysis, this mutant was found to accumulate three novel rRNA species: 23S', 18S' and 7S', each of which contains sequences from the spacer region between 25S and 18S rRNA. 35S pre-rRNA accumulates, while the level of the 20S and 27S rRNA processing intermediates is depressed. Pulse-chase analysis demonstrates that the processing of 35S pre-rRNA is slowed. The defect in the mutant appears to be at the first processing step, which generates 20S and 27S rRNA. 7S' RNA is a form of 5.8S RNA whose 5' end is extended by 149 nucleotides to a position just 5 nucleotides downstream of the normal cleavage site that produces 20S and 27S rRNA. 7S' RNA can assemble into 60S ribosomal subunits, but such subunits are relatively ineffective in joining polyribosomes. A single lesion is responsible for the pre-rRNA processing defect and the temperature sensitivity. The affected gene is designated RRP2.