Development of approaches to improve the healing following muscle contusion
Development of approaches to improve the healing following muscle contusion
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DOI：
10.1016/s0963-6897(98)00037-2
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发表时间：
1998-11-01
影响因子：
3.3
通讯作者：
Huard, J
中科院分区：
文献类型：
--
作者：
Kasemkijwattana, C;Menetrey, J;Huard, J
肌肉损伤是创伤学中一个具有挑战性的问题，也是运动医学中最常见的问题。肌肉挫伤是最常见的肌肉损伤之一。虽然这种损伤是能够愈合的，但根据钝性创伤的严重程度，通常会发生功能不完全恢复。我们开发了一种肌肉挫伤动物模型（小鼠大腿能量钝性创伤），并利用组织学和免疫组织化学表征了肌肉在损伤后的愈合能力，以确定肌肉再生水平和疤痕组织的发展。我们观察到，在损伤后的前2周发生了大量肌肉再生，随后发生了肌肉纤维化。基于这些观察结果，我们建议促进肌肉生长和再生，以及防止纤维化的发展，可以作为改善肌肉损伤愈合的方法。事实上，我们已经确定了三种生长因子（bFGF、IGF-1和NGF）能够在体外增强成肌细胞的增殖和分化，并在体内促进损伤肌肉的愈合。此外，腺病毒介导β -半乳糖苷酶直接和离体基因转移到损伤部位的能力，为这些生长因子在损伤肌肉中高效和持续表达提供了可能性。这些研究应该有助于制定策略，以促进有效的肌肉愈合和肌肉挫伤后的完全功能恢复。(C) 1998爱思唯尔科学有限公司
Muscle injuries are a challenging problem in traumatology, and the most frequent occurrence in sports medicine. Muscle contusions are among the most common muscle injuries. Although this injury is capable of healing, an incomplete functional recovery often occurs, depending on the severity of the blunt trauma. We have developed an animal model of muscle contusion in mice thigh energy blunt trauma) and characterized the muscle's ability to heal following this injury using histology and immunohistochemistry to determine the level of muscle regeneration and the development of scar tissue, We have observed a massive muscle regeneration occurring in the first 2 wk postinjury that is subsequently followed by the development of muscle fibrosis. Based on these observations, we propose that the enhancement of muscle growth and regeneration, as well as the prevention of fibrotic development, could be used as approach(es) to improve the healing of muscle injuries. In fact, we have identified three growth factors (bFGF, IGF-1, and NGF) capable of enhancing myoblast proliferation and differentiation in vitro and improving the healing of the injured muscle in vivo, Furthermore, the ability of adenovirus to mediate direct and ex vivo gene transfer of beta-galactosidase into the injured site opens possibilities of delivering an efficient and persistent expression of these growth factors in the injured muscle. These studies should help in the development of strategies to promote efficient muscle healing with complete functional recovery following muscle contusion. (C) 1998 Elsevier Science Inc.