Glycomic approach for potential biomarkers on prostate cancer: profiling of N-linked glycans in human sera and pRNS cell lines.
Glycomic approach for potential biomarkers on prostate cancer: profiling of N-linked glycans in human sera and pRNS cell lines.
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DOI：
10.1155/2008/515318
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发表时间：
2008
期刊：
影响因子：
--
通讯作者：
Lebrilla C
中科院分区：
文献类型：
--
作者：
de Leoz ML;An HJ;Kronewitter S;Kim J;Beecroft S;Vinall R;Miyamoto S;de Vere White R;Lam KS;Lebrilla C
前列腺癌是男性癌症死亡的主要原因。目前可用的筛选试验测量前列腺特异性抗原（PSA）来检测前列腺癌。然而，该测试产生假阳性值，通常导致阴性活组织检查。因此，需要一种更可靠的诊断工具。血清中的聚糖是特别有趣的，因为大约一半的蛋白质是糖基化的。在这项研究中，通过PNGase F从表达野生型或突变型雄激素受体的前列腺上皮细胞系（pRNS）和一小部分人血清样本中酶促释放n -连接聚糖。采用石墨化碳筒固相萃取（SPE）对释放的聚糖进行纯化，并将其分离为中性和酸性组分。采用基质辅助激光解吸/电离傅里叶变换离子回旋共振质谱（MALDI FT-ICR MS）对SPE组分进行分析。前列腺癌患者血清中一些高甘露糖和聚焦双触角复合物n -链聚糖的含量发生了显著变化。
Prostate cancer is a leading cause of cancer death among men. Currently available screening test measures prostate-specific antigen (PSA) to detect prostate cancer. However, this test produces false positive values that often lead to negative biopsies. Therefore, a more reliable diagnostic tool is needed. Glycans in serum are of particular interest as around half of all proteins are glycosylated. In this study, N-linked glycans were enzymatically released by PNGase F from prostate epithelial cell lines (pRNS) expressing wild type or mutant androgen receptors and a small set of human serum samples. Released glycans were purified and partitioned into neutral and acidic components by solid phase extraction (SPE) using graphitized carbon cartridges. The SPE fractions were analyzed by matrix-assisted laser desorption/ionization Fourier transform ion cyclotron resonance mass spectrometry (MALDI FT-ICR MS). Significant changes in some high-mannose and fucosylated biantennary complex N-linked glycans were observed in the serum of prostate cancer patients.
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影响因子：
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