Mechanical perturbation-induced fluorescence change of green fluorescent protein
Mechanical perturbation-induced fluorescence change of green fluorescent protein
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DOI：
10.1063/1.1856142
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发表时间：
2005-01-24
影响因子：
4
通讯作者：
Ikai, A
中科院分区：
文献类型：
--
作者：
Kodama, T;Ohtani, H;Ikai, A
原子力显微镜（AFM）的力曲线测量模式是生物技术中一种强有力的实验技术。然而，如果可以同时测量接触区域中的生物分子的光谱特性，则更有效。因此，我们开发了一个共焦激光扫描显微镜/原子力显微镜系统。在这项研究中，我们在施加外力的情况下同时测量了绿色荧光蛋白的荧光光谱，以研究β-桶结构的稳定性和动力学。因此，通过施加压缩力和拉伸力来淬灭荧光，并且在每种情况下淬灭效率不同。(C)2005年美国物理学会。
The force curve measurement mode of the atomic force microscope (AFM) is a powerful experimental technique in biotechnology. However, it is more effective if the spectroscopic properties of the biomolecule in the contact area can be simultaneously measured. Thus, we developed a confocal laser scanning microscope/AFM system. In this study, we simultaneously measured the fluorescence spectra of green fluorescent protein with the application of an external force in order to investigate the stability and dynamics of the beta-barrel structure. Consequently, the fluorescence was quenched by applying both compression and extension forces and the quenching efficiencies differed in each case. (C) 2005 American Institute of Physics.