The complete mitochondrial genome of the helmet catfish Cranoglanis bouderius (Silurifonnes: Cranoglanididae) and the phylogeny of otophysan fishes
The complete mitochondrial genome of the helmet catfish Cranoglanis bouderius (Silurifonnes: Cranoglanididae) and the phylogeny of otophysan fishes
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DOI：
10.1016/j.gene.2006.04.014
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发表时间：
2006-07-19
期刊：
影响因子：
3.5
通讯作者：
He, Shunping
中科院分区：
文献类型：
--
作者：
Peng, Zuogang;Wang, Jun;He, Shunping
采用长而准确的聚合酶链反应（LA PCR）方法测定了来自鲶鱼科Cranoglanididae的单个物种，头盔鲶鱼Cranoglanis bouderius的16，539个核苷酸的线粒体基因组的完整序列。C. bouderius的线粒体DNA已与其他三种鲶鱼在同一目的。C. bouderius线粒体基因组由13个蛋白质编码基因、2个核糖体RNA和22个转运RNA基因以及一个非编码控制区组成，其基因顺序与大多数其他脊椎动物相同。利用串联的mtDNA蛋白质编码基因序列和个体的蛋白质编码基因序列数据集，采用贝叶斯方法对13种耳鼻喉科鱼类进行系统发育分析。然后使用近似无偏检验、Kishino-Hasegawa检验和Shimodaira-Hasegawa检验来检验竞争的耳垂体拓扑结构。结果表明，分组（fonnes，Gymnotiformes），Siluriformes），Cyprinifionnes），外群）是最有可能的，但这一个和其他替代假设之间没有显着差异。此外，本文还讨论了长头鲶科在鲶形目中的系统位置。(c)2006 Elsevier B. V.保留所有权利。
The complete sequence of the 16,539 nucleotide mitochondrial genome from the single species of the catfish family Cranoglanididae, the helmet catfish Cranoglanis bouderius, was determined using the long and accurate polymerase chain reaction (LA PCR) method. The nucleotide sequences of C. bouderius mitochondrial DNA have been compared with those of three other catfish species in the same order. The contents of the C. bouderius mitochondrial genome are 13 protein-coding genes, two ribosomal RNA and 22 transfer RNA genes, and a non-coding control region, the gene order of which is identical to that observed in most other vertebrates. Phylogenetic analyses for 13 otophysan fishes were performed using Bayesian method based on the concatenated mtDNA protein-coding gene sequence and the individual protein-coding gene sequence data set. The competing otophysan topologies were then tested by using the approximately unbiased test, the Kishino-Hasegawa test, and the Shimodaira-Hasegawa test. The results show that the grouping ((((Characifonnes, Gymnotiformes), Siluriformes), Cyprinifionnes), outgroup) is the most likely but there is no significant difference between this one and the other alternative hypotheses. In addition, the phylogenetic placement of the family Cranoglanididae among siluriform families was also discussed. (c) 2006 Elsevier B.V. All rights reserved.