ALTERATION OF ANTIOXIDANT ENZYMES WITH AGING IN RAT SKELETAL-MUSCLE AND LIVER
ALTERATION OF ANTIOXIDANT ENZYMES WITH AGING IN RAT SKELETAL-MUSCLE AND LIVER
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DOI：
10.1152/ajpregu.1990.258.4.r918
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发表时间：
1990-04-01
影响因子：
--
通讯作者：
WU, E
中科院分区：
文献类型：
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作者：
JI, LL;DILLON, D;WU, E
在4月龄、26月龄和31月龄的雄性Wistar-Furth大鼠中，研究了在休息时和单次跑步机运动后骨骼肌和肝脏中参与氧自由基代谢的抗氧化酶的最大活性。在骨骼肌中，胞浆（Cu-Zn）和线粒体（Mn）超氧化物歧化酶（SOD）的比活性显着较高的老年大鼠和在31个月达到135和218%，分别为4个月。与4个月相比，31个月的静息过氧化氢酶活性增加了一倍。谷氨酰胺过氧化物酶（GPX）活性增加两倍，在肌肉细胞质和47%的线粒体老年大鼠。肌肉中谷胱甘肽S-转移酶（GST）、谷胱甘肽还原酶（GR）和葡萄糖-6-磷酸脱氢酶（G-6-PDH）的活性也显著升高。肝脏抗氧化酶的变化与年龄的差异。胞浆SOD和GST活性降低，而线粒体GPX、GR和G-6-PDH活性升高。老年大鼠骨骼肌匀浆和线粒体的脂质过氧化作用增强，而肝匀浆的脂质过氧化作用减弱。无论动物的年龄如何，急性运动对肌肉或肝脏的抗氧化酶几乎没有影响。结论：衰老伴随着骨骼肌中抗氧化酶活性和脂质过氧化的升高，可能是由于氧自由基的产生和反应增加。
Maximal activities of antioxidant enzymes involved in oxygen free radical metabolism in skeletal muscle and liver were investigated in 4-, 26-, and 31-mo-old male Wistar-Furth rat at rest and after a single bout of treadmill exercise. In skeletal muscle, cytosolic (Cu-Zn) and mitochondrial (Mn) superoxide dismutase (SOD) specific activities were significantly higher in the aged rats and at 31 mo reached 135 and 218%, respectively, of those at 4 mo. Resting catalase activity was doubled at 31 mo compared with that at 4 mo. Glutathione peroxidase (GPX) activity increased twofold in muscle cytosol and by 47% in mitochondria of aged rats. Glutathione S-transferase (GST), glutathione reductase (GR), and glucose-6-phosphate dehydrogenase (G-6-PDH) activities in muscle were also significantly elevated. Hepatic antioxidant enzymes were altered differentially with aging. Cytosolic SOD and GST activities were decreased, whereas mitochondrial GPX, GR, and G-6-PDH activities were increased. Lipid peroxidation was greater in skeletal muscle homogenate and mitochondria but lower in liver homogenate in the aged rats. An acute exercise bout had little effect on muscle or liver antioxidant enzymes regardless of the animal's age. It is concluded that aging is accompanied with an elevation of antioxidant enzyme activities and lipid peroxidation in skeletal muscle probably due to the increased oxygen free radical production and reaction.