Environmental Oxygen Exposure Allows for the Evolution of Interdigital Cell Death in Limb Patterning
Environmental Oxygen Exposure Allows for the Evolution of Interdigital Cell Death in Limb Patterning
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DOI：
10.1016/j.devcel.2019.05.025
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发表时间：
2019-07-22
影响因子：
11.8
通讯作者：
Tanaka, Mikiko
中科院分区：
文献类型：
--
作者：
Cordeiro, Ingrid Rosenburg;Kabashima, Kaori;Tanaka, Mikiko
两栖动物通过趾和趾间区域的差异生长形成没有蹼的手指。然而，羊膜动物采用趾间细胞死亡（ICD），这是一种额外的机制，有助于肢体形状的更大变化。在这里，我们调查的作用，环境中的氧气在四足动物ICD的演变。虽然细胞死亡仅限于两栖动物的四肢边缘与水生蝌蚪，Eleutherodactylus coqui，青蛙与地面直接发展的鸡蛋，有细胞死亡的趾间区。鸡需要足够的氧和活性氧来诱导细胞死亡，鸡和非洲爪蟾蛙的四肢之间的氧张力曲线本身是不同的。值得注意的是，增加血管密度在X。leevis肢，以及在高氧水平下孵化蝌蚪，诱导ICD。我们认为，氧气提供给陆地鸡蛋是一个生态功能至关重要的ICD的演变，可能是由保守的autopod模式化机制。
Amphibians form fingers without webbing by differential growth between digital and interdigital regions. Amniotes, however, employ interdigital cell death (ICD), an additional mechanism that contributes to a greater variation of limb shapes. Here, we investigate the role of environmental oxygen in the evolution of ICD in tetrapods. While cell death is restricted to the limb margin in amphibians with aquatic tadpoles, Eleutherodactylus coqui, a frog with terrestrial-direct-developing eggs, has cell death in the interdigital region. Chicken requires sufficient oxygen and reactive oxygen species to induce cell death, with the oxygen tension profile itself being distinct between the limbs of chicken and Xenopus laevis frogs. Notably, increasing blood vessel density in X. laevis limbs, as well as incubating tadpoles under high oxygen levels, induces ICD. We propose that the oxygen available to terrestrial eggs was an ecological feature crucial for the evolution of ICD, made possible by conserved autopod-patterning mechanisms.