BS69 cooperates with TRAF3 in the regulation of Epstein-Barr virus-derived LMP1/CTAR1-induced NF-κB activation
BS69 cooperates with TRAF3 in the regulation of Epstein-Barr virus-derived LMP1/CTAR1-induced NF-κB activation
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DOI：
10.1016/j.febslet.2010.01.060
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发表时间：
2010-03-05
期刊：
影响因子：
3.5
通讯作者：
Matsuda, Tadashi
中科院分区：
文献类型：
--
作者：
Ikeda, Osamu;Miyasaka, Yuto;Matsuda, Tadashi
EB病毒潜伏膜蛋白1（LMP 1）通过两个C末端区域CTAR 1和CTAR 2激活NF-κ B信号通路。先前的研究表明，BS 69，一种多结构域细胞蛋白，通过干扰TRADD和LMP 1/CTAR 2之间的复合物形成来调节LMP 1/CTAR 2介导的NF-κ B活化。在这里，我们发现BS 69直接与LMP 1/CTAR 1结构域相互作用，并调节LMP 1/CTAR 1介导的NF-κ B活化和随后的IL-6产生。关于所涉及的机制，我们发现BS 69直接与TRAF 3相互作用，TRAF 3是NF-κ B激活的负调节因子。此外，小干扰RNA介导的敲低实验揭示TRAF 3参与BS 69介导的对LMP 1/CTAR 1诱导的NF-κ B活化的抑制。（uniprotkb：P03230）与BS 69发生物理相互作用（MI：0915）（uniprotkb：Q15326）通过抗标签共免疫沉淀（MI：0007）MINT-7556646：TRAF6（uniprotkb：Q9 Y 4K 3）物理相互作用（MI：0915），带BS 69（uniprotkb：Q15326）通过抗标签共免疫沉淀（MI：0007）MINT-7556658，MINT-7556670：TRAF3（uniprotkb：Q13114）物理相互作用（MI：0915）带BS 69（uniprotkb：Q15326）通过抗标签共免疫沉淀（MI：0007）MINT-7556607：TRAF1（uniprotkb：Q13077）物理相互作用（MI：0915）带BS 69（uniprotkb：Q15326）通过抗标签共免疫沉淀（MI：0007）MINT-7556634：TRAF5（uniprotkb：O 00463）物理相互作用（MI：0915），带BS 69（uniprotkb：Q15326）通过抗标签共免疫沉淀（MI：0007）MINT-7556622：TRAF2（uniprotkb：Q12933）物理相互作用（MI：0915）与BS 69（uniprotkb：Q15326）通过抗标签共免疫沉淀（MI：0007）（C）2010欧洲生物化学学会联合会。由Elsevier B出版。V.保留所有权利。
Epstein-Barr virus latent membrane protein 1 (LMP1) activates NF-kappa B signaling pathways through two C-terminal regions, CTAR1 and CTAR2. Previous studies have demonstrated that BS69, a multidomain cellular protein, regulates LMP1/CTAR2-mediated NF-kappa B activation by interfering with the complex formation between TRADD and LMP1/CTAR2. Here, we found that BS69 directly interacted with the LMP1/CTAR1 domain and regulated LMP1/CTAR1-mediated NF-kappa B activation and subsequent IL-6 production. Regarding the mechanisms involved, we found that BS69 directly interacted with TRAF3, a negative regulator of NF-kappa B activation. Furthermore, small-interfering RNA-mediated knockdown experiments revealed that TRAF3 was involved in the BS69-mediated suppression of LMP1/CTAR1-induced NF-kappa B activation.Structured summary:MINT-7556591: lmp1 (uniprotkb: P03230) physically interacts (MI:0915) with BS69 (uniprotkb:Q15326) by anti tag coimmunoprecipitation (MI: 0007)MINT-7556646: TRAF6 (uniprotkb:Q9Y4K3) physically interacts (MI: 0915) with BS69 (uniprotkb: Q15326) by anti tag coimmunoprecipitation (MI: 0007)MINT-7556658, MINT-7556670: TRAF3 (uniprotkb:Q13114) physically interacts (MI: 0915) with BS69 (uniprotkb: Q15326) by anti tag coimmunoprecipitation (MI: 0007)MINT-7556607: TRAF1 (uniprotkb:Q13077) physically interacts (MI: 0915) with BS69 (uniprotkb: Q15326) by anti tag coimmunoprecipitation (MI: 0007)MINT-7556634: TRAF5 (uniprotkb:O00463) physically interacts (MI: 0915) with BS69 (uniprotkb: Q15326) by anti tag coimmunoprecipitation (MI: 0007)MINT-7556622: TRAF2 (uniprotkb:Q12933) physically interacts (MI: 0915) with BS69 (uniprotkb: Q15326) by anti tag coimmunoprecipitation (MI: 0007) (C) 2010 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.