Trade-offs constrain adaptive pathways to the type VI secretion system survival.
Trade-offs constrain adaptive pathways to the type VI secretion system survival.
复制标题
权衡限制适应途径VI型分泌系统的生存。
DOI：
10.1016/j.isci.2023.108332
复制
发表时间：
2023-12-15
期刊：
影响因子：
5.8
通讯作者：
Hammer, Brian K.
中科院分区：
文献类型：
--
作者：
Macgillivray, Kathryn A.;Ng, Siu Lung;Wiesenfeld, Sophia;Guest, Randi L.;Jubery, Tahrima;Silhavy, Thomas J.;Ratcliff, William C.;Hammer, Brian K.
VI型分泌系统（T6 SS）是许多细菌用来将毒素注入邻近细胞的纳米鱼叉。虽然对T6 SS介导的毒性机制了解很多，但对竞争对手如何保护自己免受这种攻击知之甚少，特别是在缺乏自己的T6 SS的情况下。在这里，我们进行了8个重复的人口大肠杆菌T6 SS攻击霍乱弧菌。在超过1500代的竞争中，大肠杆菌种群的分离株通过两种趋同进化途径进化为平均27倍更好地在T6 SS攻击中生存：apaH在8个重复种群中的6个中发生突变，而另外两个种群各自在yejM和yjeP中发生突变。然而，我们发现的突变是多效性的，降低了细胞生长速率，增加了对抗生素和pH值升高的敏感性。这些权衡有助于我们了解T6 SS如何塑造细菌相互作用的演变。500代后大肠杆菌对霍乱弧菌T6 SS的抗性提高了27倍趋同进化揭示了提高T6 SS存活率的突变途径机械敏感通道基因突变提供了对T6 SS的广谱抗性提高T6 SS存活率的突变减少了生长，揭示了进化的权衡细菌学;生物科学;进化机制
The Type VI Secretion System (T6SS) is a nano-harpoon used by many bacteria to inject toxins into neighboring cells. While much is understood about mechanisms of T6SS-mediated toxicity, less is known about the ways that competitors can defend themselves against this attack, especially in the absence of their own T6SS. Here we subjected eight replicate populations of Escherichia coli to T6SS attack by Vibrio cholerae. Over ∼500 generations of competition, isolates of the E. coli populations evolved to survive T6SS attack an average of 27-fold better, through two convergently evolved pathways: apaH was mutated in six of the eight replicate populations, while the other two populations each had mutations in both yejM and yjeP. However, the mutations we identified are pleiotropic, reducing cellular growth rates, and increasing susceptibility to antibiotics and elevated pH. These trade-offs help us understand how the T6SS shapes the evolution of bacterial interactions. E. coli evolved 27-fold higher resistance to V. cholerae T6SS after 500 generations Convergent evolution reveals just mutational pathways for improving T6SS survival A mechanosensitive channel gene mutation provides broad spectrum resistance to T6SS Mutations improving T6SS survival reduce growth, revealing an evolutionary tradeoff Bacteriology; Biological sciences; Evolutionary mechanisms
登录
查看更多内容
影响因子：
16.6
作者：
Flaugnatti N;Isaac S;Lemos Rocha LF;Stutzmann S;Rendueles O;Stoudmann C;Vesel N;Garcia-Garcera M;Buffet A;Sana TG;Rocha EPC;Blokesch M
通讯作者：
Blokesch M
影响因子：
3.5
作者：
CHEREPANOV, PP;WACKERNAGEL, W
通讯作者：
WACKERNAGEL, W
影响因子：
5.4
作者：
Fischbach, Michael A.
通讯作者：
Fischbach, Michael A.
影响因子：
3.7
作者：
Alteri CJ;Mobley HLT
通讯作者：
Mobley HLT
影响因子：
5.2
作者：
Gorter FA;Tabares-Mafla C;Kassen R;Schoustra SE
通讯作者：
Schoustra SE