TEMPORAL-SPATIAL DISTRIBUTION OF SP-B AND SP-C PROTEINS AND MESSENGER-RNAS IN DEVELOPING RESPIRATORY EPITHELIUM OF HUMAN LUNG
TEMPORAL-SPATIAL DISTRIBUTION OF SP-B AND SP-C PROTEINS AND MESSENGER-RNAS IN DEVELOPING RESPIRATORY EPITHELIUM OF HUMAN LUNG
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DOI：
10.1177/42.9.8064126
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发表时间：
1994-09-01
影响因子：
3.2
通讯作者：
WHITSETT, JA
中科院分区：
文献类型：
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作者：
KHOOR, A;STAHLMAN, MT;WHITSETT, JA
我们通过免疫组织化学和原位杂交方法确定了表面活性蛋白B（前体SP - B）和C（前体SP - C）的mRNA及蛋白质在胎儿、新生儿和成人肺中的时空分布。在妊娠15周时，可在支气管和细支气管中检测到前体SP - B和SP - B的mRNA。25周后，前体SP - B、有活性的SP - B肽以及SP - B的mRNA共同定位于细支气管 - 肺泡门细胞和Ⅱ型上皮细胞中。在成人肺中，前体SP - B和SP - B的mRNA主要在无纤毛细支气管上皮细胞和肺泡中的Ⅱ型细胞中被检测到。从妊娠15周起及之后，在终末气道的内衬细胞中可检测到前体SP - C和SP - C的mRNA。25周后，在细支气管 - 肺泡门的上皮细胞和Ⅱ型细胞中可检测到SP - C的mRNA和前体蛋白，且其表达随着胎龄的增加而增加。与有活性的SP - B肽相比，前体SP - B独特的细胞染色模式支持了这样一种观点，即其蛋白水解加工或细胞转运可能受到细胞类型和/或细胞分化的影响。SP - B和SP - C在人胎儿肺的远端传导气道和终末气道上皮中表达，远远早于表面活性脂质合成或出生时产生肺表面活性物质的生理需求。
We determined the temporal and spatial distribution of surfactant protein B (pro-SP-B) and C (pro-SP-C) mRNAs and proteins by immunohistochemistry and in situ hybridization in fetal, neonatal, and adult human lung. Pro-SP-B and SP-B mRNA were detected in bronchi and bronchioles by 15 weeks' gestation. After 25 weeks, pro-SP-B, active SP-B peptide, and SP-B mRNA were co-localized in bronchiolo-alveolar portal cells and in Type II epithelial cells. In adult lung, pro-SP-B and SP-B mRNA were detected primarily in non-ciliated bronchiolar epithelial cells and in Type II cells in the alveolus. Pro-SP-C and SP-C mRNA were detected in cells lining terminal airways from 15 weeks' gestation and thereafter. After 25 weeks, SP-C mRNA and precursor protein were detected in epithelial cells of the bronchiolo-alveolar portals and in Type II cells, where expression increased with advancing gestational age. Distinct cellular patterns of staining for pro-SP-B compared with SP-B active peptide support the concept that its proteolytic processing or cellular routing may be influenced by cell type and/or cell differentiation. SP-B and SP-C are expressed primarily in distal conducting and terminal airway epithelium of human fetal lung well in advance of surfactant lipid synthesis or physiologic requirements to produce pulmonary surfactant at the time of birth.