Whole cell nuclear magnetic resonance characterization of two photochemically active states of the photosynthetic reaction center in heliobacteria.
Whole cell nuclear magnetic resonance characterization of two photochemically active states of the photosynthetic reaction center in heliobacteria.
复制标题
日光细菌光合反应中心两种光化学活性状态的全细胞核磁共振表征。
作者：
Smitha Surendran Thamarath;A. Alia;Eugenio Daviso;D. Mance;J. Golbeck;J. Matysik
应用光化学诱导动态核极化（Photo-CIDNP，Photochemically Induced Dynamic Nuclear Polarization）MAS（magic-anglespinning）核磁共振技术对太阳杆菌（Heliobacillus，Hb.）mobilis，我们表明，太阳菌反应中心是在两个不同的状态下操作所示的光诱导的自旋相关的自由基对的发生。在厌氧条件下培养的培养物被称为“布朗斯托夫”（德语为“棕色物质”）。暴露于氧气后，Braunstoff转化为“Grünstoff”（“绿色物质”），如由于BChl g转化为Chl a（F）而引起的颜色变化所示。结果表明，电子转移对称地发生通过两个分支的辅因子在两种形式。供体和受体辅因子在转化后保持相同且不变，而中间辅助辅因子从BChl g转化为Chl a（F）。Braunstoff中的供体三重态位于特殊的对供体上，并存活100 μs，表明附近没有类胡萝卜素。在Grünstoff中，供体三联体变得移动的，似乎是在辅助辅因子上形成的。
Applying photo-CIDNP (photochemically induced dynamic nuclear polarization) MAS (magic-angle spinning) nuclear magnetic resonance to whole cells of Heliobacillus (Hb.) mobilis, we demonstrate that heliobacterial reaction centers are operational in two different states as indicated by the occurrence of a light-induced spin-correlated radical pair. A culture maintained anaerobically is called "Braunstoff" (German for "brown substance"). After exposure to oxygen, Braunstoff is converted to "Grünstoff" ("green substance") as indicated by a color change due to the conversion of BChl g to Chl a(F). It is shown that electron transfer occurs symmetrically via both branches of cofactors in both forms. The donor and acceptor cofactors remain identical and unchanged upon conversion, while the intermediate accessory cofactors are transformed from BChl g to Chl a(F). The donor triplet state in Braunstoff is localized on the special pair donor and lives for 100 μs, demonstrating the absence of nearby carotenoids. In Grünstoff, the donor triplet becomes mobile and appears to be formed on an accessory cofactor.
通过 (13)C 魔角旋转 NMR 光谱观察趋光素 LOV1-C57S 中的固态光 CIDNP 效应
影响因子：
15
作者：
S. S. Thamarath;J. Heberle;P. Hore;T. Kottke;J. Matysik
通讯作者：
J. Matysik