Identification of a novel Mannheimia granulomatis lineage from lesions in roe deer (Capreolus capreolus)
Identification of a novel Mannheimia granulomatis lineage from lesions in roe deer (Capreolus capreolus)
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DOI：
10.7589/0090-3558-43.3.345
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发表时间：
2007-07-01
影响因子：
1.3
通讯作者：
Bisgaard, Magne
中科院分区：
文献类型：
--
作者：
Bojesen, Anders M.;Larsen, Jesper;Bisgaard, Magne
从鹿病（Capreolus Capreolus Capreolus）中分离出8株非典型曼海姆病分离株。基于形态和生理特征的传统分类表明，它们属于Mannheimia属内一个独特的生物群（分类群）。广泛的表型特征表明，分离物应分类为肉芽肿支原体，尽管存在明显的性状证明它们被分类为该物种内一个单独的生物群。基于2株鹿分离株和41株曼海姆氏菌的16S rRNA序列的系统发育分析支持了该分离株在肉芽肿支原体中形成一个单系群。根据Southern blot分析，所有的狍分离株中都存在lktA基因型，而其中一个分离株中没有相应的(3)溶血表型。
Eight atypical Mannheimia isolates were isolated from lesions in roe deer (Capreolus capreolus). Traditional classification based on morphologic and physiologic traits showed that they belong to a distinct biogroup (taxon) within genus Mannheimia. Extensive phenotypic characterization suggested that the isolates should be classified as M. granulomatis, although the presence of distinct traits justified their classification into a separate biogroup within this species. Phylogenetic analyses based on 16S rRNA sequences from two roe deer isolates and 41 other Mannheimia strains supported that the roe deer isolates form a monophyletic group within M. granulomatis. The lktA genotype was present in all roe deer isolates based on Southern blot analysis, whereas the corresponding (3-hemolytic phenotype was absent in one of these isolates.